Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Test material form:
liquid
Details on test material:
dark clear liquid

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no
Details on test solutions:
Due to the lack of toxicity observed in preliminary range-finding tests (0.01 to 1000 mg/l concentrations tested) OECD Limit Tests were conducted using direct addition of material.
100 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
SPECIES: Daphnia magna
ACCLIMATION/TEST WATER: Hard Syntetic Freshwater
FEEDING PRIOR TO TEST: YCT + Selenastrum capricornutum
FEEDING DURING TEST: None -YCT + Selenastrum 2+ h before test
SOURCE: CI Stock cultures
ACCLIMATION TEMP (°C ): 20
BROOD RELEASE START DATE & TIME: 3/30/18 16:00
BROOD RELEASE END DATE & TIME: 3/31/18 10:30
DATE/TIME WATER ADDED: 3/31/18 13:59
DATE/TIME ANIMALS ADDED: 3/31/18 14:17
ANIMAL AGE WINDOW: 18h 30m
MAX AGE AT TEST START: 22h 17m

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
19 - 21
pH:
8.00 - 8.28
Dissolved oxygen:
7.2 - 8.9 mg/L
Conductivity:
568 - 579 uS/cm
Reference substance (positive control):
yes
Remarks:
KCl

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 was greater than the test concentration of 100 mg/l.