(S)-2-chloropropionic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

19th - 23rd October 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was performed to GLP and in accordance with OECD guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(S)-2-chloropropionic acid

EC Number:

411-150-5

EC Name:

(S)-2-chloropropionic acid

Cas Number:

29617-66-1

Molecular formula:

Hill formula: C3H5ClO2 CAS formula: C3H5ClO2

IUPAC Name:

(2S)-2-chloropropanoic acid

Details on test material:

- Name of test material (as cited in study report): L-2-chloropropionic acid referred to throughout the study as W717.
- Physical state: Liquid
- Analytical purity: 90.4 %w/w
- Purity test date: 30th April 1992
- Lot/batch No.: Bx 0071, ADH05138
- Expiration date of the lot/batch: Not specified
- Stability under test conditions: Not specified
- Storage condition of test material: Ambient temperature in original container.

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

No signs of toxicity were observed in any of the test fish at the daily observation times.

The mortality results are summarised in table 1.

 

Table 1.

Time (hours)	Nominal Concentration W717 (mg/L)	Number Tested	Number Dead	% Dead
24	Control	10	0	0
	1000	10	0	0
48	Control	10	0	0
	1000	10	0	0
72	Control	10	0	0
	1000	10	0	0
96	Control	10	0	0
	1000	10	0	0

 

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

After 96 hours there were no mortalities in either the test concentration or the control vessel. The LC50 can be defined as > 1000mg/L W717(L-2-chloropropionic acid).

Executive summary:

The toxicity of L-2 -chloropropionic acid to fish was studied on rainbow trout (Oncorhynchus mykiss) in a 96 hour static study according to OECD 203.

L-2-chloropropionic acid 90.4% pure was used at nominal concentrations of 0 and 1000 mg/l. The test concentration was verified by chemical analysis. Ten fish with a mean length of 4.5 cm and mean weight of 1.29 g were exposed to each test concentration. The test media pH was adjusted to pH 7.6 prior to the addition of the fish, the temperature was maintained at 15+/-1^oC and the light regime was fluorescent light for 16 hours and darkness for 8 hours. The fish were not fed throughout the test period.

Observations for mortality or toxicity in the fish were made at 24, 48, 72 and 96 hours during the test.

After 96 hours no mortalities were observed in the test concentration or in the control vessel.

The LC50, defined as the concentration resulting in the death of 50% of the fish in the time period specified, was therefore >1000mg/L.