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EC number: 411-150-5 | CAS number: 29617-66-1 L-2-CHLOROPROPIONIC ACID; L-2-CHLORPROPIONSÄURE
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2nd - 30th June 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was performed in accordance with GLP and to relevant guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- yes
- Remarks:
- The test solutions were not analysed on the last two consecutive days of the test as stated in the guideline.
- Principles of method if other than guideline:
- The test solutions were not analysed on the last two consecutive days of the test as stated in the guideline.
This was considered not to have adversely affected the results of the study.
The guideline also states that the Zahn-Wellen units should be topped up with water to replace that lost through evaporation but that account should be taken of the reduction in volume due to the removal of samples. In this study the volume was made up to the original volume of 1.5 litres, resulting in a gradual dilution of the unit contents over the course of the study. As a result the unit contents will be 89% of the starting concentrations. Given the results of the functional control (100% biodegradation in 5 days) this was considered not to adversely affect the results of the study. - GLP compliance:
- yes
Test material
- Reference substance name:
- (S)-2-chloropropionic acid
- EC Number:
- 411-150-5
- EC Name:
- (S)-2-chloropropionic acid
- Cas Number:
- 29617-66-1
- Molecular formula:
- Hill formula: C3H5ClO2 CAS formula: C3H5ClO2
- IUPAC Name:
- (2S)-2-chloropropanoic acid
- Details on test material:
- - Name of test material (as cited in study report): L-2-Chloropropionic acid, referred to as AB0350 throughout the study.
- Physical state: Liquid
- Analytical purity: 92.7% w/w
- Purity test date: 18th April 1995
- Lot/batch No.: Bx 19
- Expiration date of the lot/batch: Not specified
- Storage condition of test material: ambient temperature
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Buckland Sewage Treatment Works, (Newton Abbot , Devon)
- Storage conditions: Aerated at room temperature, pH maintained at pH 7.
- Preparation of inoculum for exposure: On the day of the test the activated sludge was washed twice by allowing the sludge to settle, removing the supernatant liquid and making up to the original volume by the addition of water.
- Initial cell/biomass concentration: solids concentration of 2477 mg/L
- Water filtered: no data - Duration of test (contact time):
- ca. 28 d
Initial test substance concentration
- Initial conc.:
- ca. 1 000 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- other: Dissolved non-purgeable organic carbon analysis (DNPOC)
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral nutrient solution was prepared according to the 'Official Jounal of C, L133, Volume 31 Part C. Methods for determination of ecotoxicity. 30 May 1988. The composition was as follows, per litre of deionised water.
Ammonium chloride: 38.5g
Sodium dihydrogen phosphate: 33.4g
potassium dihydrogen phosphate: 8.5g
dipotassium hydrogen phosphate: 21.75g
- Solubilising agent : Not required
- Test temperature: 20 +/- 3oC
- pH: pH 7.0 - 7.6
- pH adjusted: yes if required
- Aeration of dilution water: Not specified
- Suspended solids concentration: Not specified
- Continuous darkness:Not specified
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Not specified
- Test performed in open system: yes
SAMPLING
- Sampling frequency: 3 hours, days 5, 7, 11, 14, 21, 28.
- Sampling method: 25ml aliquots were removed from the test vessel.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- other: Dissolved Non Purgeable Organic Carbon
- Value:
- ca. 100
- Sampling time:
- 21 d
Any other information on results incl. tables
The resuts are summarised in the table below.
Dissolved Non Purgeable Organic Carbon Results
Test time |
Blank DNPOC (mg/L) |
AB0350 (LCPA) |
Aniline |
||||
|
|
DNPOC (mg/L) |
Net DNPOC (mg/L) |
Biodegradation *(%) |
DNPOC (mg/L) |
Net DNPOC (mg/L) |
Biodegradation *(%) |
3 hours |
5.7 |
42 |
36 |
<1 |
79 |
73 |
<1 |
5 days |
19 |
52 |
33 |
9 |
9 |
<1 |
100 |
7 days |
24 |
67 |
43 |
<1 |
15 |
<1 |
100 |
11 days |
18 |
20 |
2 |
94 |
11 |
<1 |
100 |
14 days |
19 |
20 |
2 |
96 |
15 |
<1 |
100 |
21 days |
16 |
15 |
<1 |
100 |
10 |
<1 |
100 |
28 days |
17 |
16 |
<1 |
100 |
15 |
<1 |
100 |
The DNPOC results are the mean of duplicate analyses.
Where the calculation of % biodegradation resolves as zero or as a negative figure this is reported as <1.
Where the calculation of net DNPOC resolves as a negative, this is reported as <1.
Where the calculation of % biodegradation resolves as more than 100% this is reported as 100% biodegradation.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- The results for AB0350 (LCPA) show that there was an adaptation period phase of at least 7 days and that by day 11 of the test 90% degradation was observed. Complete biodegradation was obserevd by day 21. Therefore the conclusion is that the substance is inherently biodegradable.
- Executive summary:
A guideline GLP study was conducted to assess the inherent biodegradability of L-2-Chloropropionc acid using a Zahn-Wellens test over a 28 day period. Activated sludge obtained from the local sewage works was the inoculum.
The concentration of dissolved non purgeable organic carbon was used to assess the ultimate biodegradation over the test period; the concentration of L-2-chloropropionic acid was determined by HPLC at the end of the test period to assess any primary biodegradation that may have occurred.
An aqueous mixture of activated sludge, mineral nutrients and test material was placed in a cylindrical vessel with stirring and aeration for 28 days. A reference substance (aniline) and blank solutions were also tested concurrently. All tests were performed in duplicate.
The nominal concentration for the test substance and reference substance was 1000 mg/L.
Samples were removed from the test solutions at 3 hours and days 5, 7, 11, 14, 21 and 28.
A 25 ml sample was removed, centrifuged and the supernatant liquid analysed for DNPOC. The pH of each test vessel was monitored daily and adjusted if outside the specified limits of pH 7.0 -7.6. Any loss in volume due to evaporation was made up by the addition of water.
The results showed there was an adaptation phase of at least 7 days and that by day 11 of the test there was > 90% degradation. Complete biodegradation had taken place by day 21. The conclusion is that L-2-chloropropionic acid is inherently biodegradable.
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