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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08 May - 07 Jun, 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: done under OECD method and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
Version / remarks:
Part IV
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
424-660-8
EC Name:
-
Cas Number:
224631-15-6
Molecular formula:
Hill formula: C18H26N4O5S CAS formula: C18H26N4O5S
IUPAC Name:
(S)-2,5-dioxopyrrolidin-1-yl 2-(3-((2-isopropylthiazol-4-yl)methyl)-3-methylureido)-3-methylbutanoate

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
29 d
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
A pre-determined amount of test material was added to a liquid medium to give duplicate bioreactors containing 10mg and 20 mg Organic Carbon (OC). These were inoculated with micro-organisms from activated sludge and aerated at 20-25°C. A ny Co2 released as a result of biodegredation trapped and monitored over 29 days.. The total CO2 produced was calculated as a percentage of the total CO2 that the test material,, based on its carbon content, could have theoretically produced.
Reference substance
Reference substance:
other: Sodium benzoate

Results and discussion

Preliminary study:
Under the strict conditions of the test, A-133816.0 is determined not to be readily biodegadeable. A-133816.0 is determined to be readily biodegadable at a concentration of 20mg/L
There was no inhibitory effects to the micro-organisms contained in the sludge innoculum at a concentration of ca. 37.8mg/L
% Degradationopen allclose all
Parameter:
% degradation (CO2 evolution)
Value:
20.83
Sampling time:
20 d
Remarks on result:
other: 10mg/l sample
Parameter:
% degradation (CO2 evolution)
Value:
51.18
Sampling time:
20 d
Remarks on result:
other: 20mg/L sample

BOD5 / COD results

Results with reference substance:
Sodium benzoate attained 76.16% biodegredation within 20 days

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other:
Conclusions:
Under the strict conditions of the test, A-133816.0 is determined not to be readily biodegadeable. A-133816.0 is determined to be readily biodegadable at a concentration of 20mg/L
There was no inhibitory effects to the micro-organisms contained in the sludge innoculum at a concentration of ca. 37.8mg/L