Registration Dossier
Registration Dossier
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EC number: 424-660-8 | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 May - 07 Jun, 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: done under OECD method and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Version / remarks:
- Part IV
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 424-660-8
- EC Name:
- -
- Cas Number:
- 224631-15-6
- Molecular formula:
- Hill formula: C18H26N4O5S CAS formula: C18H26N4O5S
- IUPAC Name:
- 2,5-dioxopyrrolidin-1-yl (2S)-3-methyl-2-{[methyl({[2-(propan-2-yl)-1,3-thiazol-4-yl]methyl})carbamoyl]amino}butanoate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Duration of test (contact time):
- 29 d
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- A pre-determined amount of test material was added to a liquid medium to give duplicate bioreactors containing 10mg and 20 mg Organic Carbon (OC). These were inoculated with micro-organisms from activated sludge and aerated at 20-25°C. A ny Co2 released as a result of biodegredation trapped and monitored over 29 days.. The total CO2 produced was calculated as a percentage of the total CO2 that the test material,, based on its carbon content, could have theoretically produced.
Reference substance
- Reference substance:
- other: Sodium benzoate
Results and discussion
- Preliminary study:
- Under the strict conditions of the test, A-133816.0 is determined not to be readily biodegadeable. A-133816.0 is determined to be readily biodegadable at a concentration of 20mg/L
There was no inhibitory effects to the micro-organisms contained in the sludge innoculum at a concentration of ca. 37.8mg/L
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 20.83
- Sampling time:
- 20 d
- Remarks on result:
- other: 10mg/l sample
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 51.18
- Sampling time:
- 20 d
- Remarks on result:
- other: 20mg/L sample
BOD5 / COD results
- Results with reference substance:
- Sodium benzoate attained 76.16% biodegredation within 20 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other:
- Conclusions:
- Under the strict conditions of the test, A-133816.0 is determined not to be readily biodegadeable. A-133816.0 is determined to be readily biodegadable at a concentration of 20mg/L
There was no inhibitory effects to the micro-organisms contained in the sludge innoculum at a concentration of ca. 37.8mg/L
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