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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 November 2010 - 24 January 2011
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1,2-bis(2-methylpropyl) (1R,2R,3R,6S)-3,6-dimethylcyclohexane-1,2-dicarboxylate; 1,2-bis(2-methylpropyl) (1S,2S,3R,6S)-3,6-dimethylcyclohexane-1,2-dicarboxylate
EC Number:
943-279-2
Molecular formula:
C18H32O4
IUPAC Name:
1,2-bis(2-methylpropyl) (1R,2R,3R,6S)-3,6-dimethylcyclohexane-1,2-dicarboxylate; 1,2-bis(2-methylpropyl) (1S,2S,3R,6S)-3,6-dimethylcyclohexane-1,2-dicarboxylate
Test material form:
liquid
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: KM0901D
- Expiration date of the lot/batch:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: dark storage place at room temperature
- Stability: confirmed by comparison of IR spectrums before and after the test
- Stability under test conditions: prior to the biovocentration test, a stability of the test item under the testing conditions was confirmed by a preliminary test.

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Carp
- Age: Yeasrling fish
- Length at study initiation: 6.6 - 8.7 cm
- Lipid content at test initiation (mean and range, SD):
- Feeding during test
- Food type: feed for fry of carp
- Amount: 3% of total body weight
- Frequency: twice a day
- The fish are starved for 24 hours before sampling

ACCLIMATION
- Acclimation period: 20 days
- Acclimation conditions (same as test or not): 25 +/- 2°C
- Health during acclimation (any mortality observed): A mortality during acclimatization was less then 5%

Study design

Test type:
flow-through
Water / sediment media type:
natural water
Total exposure / uptake duration:
28 d

Test conditions

Test temperature:
24.5 - 25.0 °C
pH:
8.1
Dissolved oxygen:
7.7 - 8.0 mg/L

Results and discussion

Lipid contentopen allclose all
Lipid content:
4.23 %
Time point:
start of exposure
Lipid content:
4.12 %
Time point:
end of exposure
Bioaccumulation factoropen allclose all
Conc. / dose:
10 µg/L
Temp.:
25 °C
Type:
BCF
Value:
170 dimensionless
Time of plateau:
28 d
Calculation basis:
steady state
Conc. / dose:
1 µg/L
Temp.:
25 °C
Type:
BCF
Value:
160 dimensionless
Time of plateau:
28 d
Calculation basis:
steady state

Applicant's summary and conclusion

Conclusions:
No abnormality in behavior or appearance was noted.