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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 April 2017 to 1 June 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- excess test solutions were sampled and at the end of the test one replicate of the dilution water control, solvent control and each test concentration was sampled
- Concentrations: all concentrations
- Sampling method: from excess test solutions at the start of the test and from one replicate of the dilution water control, solvent control and each test concentration at the end of the test
- Sample storage conditions before analysis: no data
At the end of the tests all test concentration vessels were rinsed with 20 ml of 0.5 mg/l Phenanthrene in hexane and an aliquot transferred to an auto-sampler vial for analysis - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION A primary stock concentrate of the substance with a nominal concentration of 500 g/L, was prepared by weighing a nominal 5 g of test substance and making up to 10 mL with the solvent tetrahydrofuran (THF) in a volumetric flask. The resultant stock was observed to be a clear and colourless solution and was used to prepare the test solutions. THF concentration in controls and exposure solutions was 0.1 mg/L.
Test solutions at 0.3125, 0.625, 1.25 mg/Lwere clear at t=0, but at 1.25 mg/L globules of the substance on the surface were seen at 24 and 48 hours, Test solutions at 2.5 and 5.0 mg/L contained globules of the substance on the surface at all obeservation times. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: laboratory cultures held at Scymaris
- Age of parental stock (mean and range, SD): 12 ± 1 day
- Feeding during test: none
ACCLIMATION
- Acclimation period: NA
- Stock conditions (same as test or not): in a reconstituted water medium, the same as the test dilution water, at a temperature of 20 ± 2°C.
- Type and amount of food: mixed algae diet of Chlorella vulgaris and Pseudokirchneriella subcapitata
- Feeding frequency: daily
- Health during acclimation: no evidence of disease:
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 230 mg/L as CaCO3
- Test temperature:
- 19.7 - 20.5 °C
- pH:
- 7.8-7.9
- Dissolved oxygen:
- 9.0-9.2 mg/L
- Conductivity:
- 620 µS/cm
- Nominal and measured concentrations:
- nominal: 0, 0.3125, 0.625, 1.25, 2.5 and 5.0 mg/L
measured: LOQ, 0.16, 0.44, 0.62, 1.4 and 2.1 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass beaker with 200 mL test solution
- Type: closed (loss fitting glass lid)
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per vehicle control (replicates):4
TEST MEDIUM / WATER PARAMETERS: ELENDT M4 reconstituted water according to the guideline
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 h light/8 h dark
EFFECT PARAMETERS MEASURED: immobility at 24 and 48 hours
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY:not performed - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Details on results:
- No immobility was observed in any of the test vessels at any concentration tested,
The recovery of the substance after the rinsing of the test vessels after 48 hours was 34-54% of the initial loading - Reported statistics and error estimates:
- NA
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 for daphnia is > 2.1 mg/L (measured concentration)
- Executive summary:
In a test according to OECD 202, 20 daphnids/concentration were exposed to 0, 0.3125, 0.625, 1.25, 2.5 and 5.0 mg/L of the substance. In the test solutions 0.1 mg/L of the solvent THF was present. No immobility was observed in any of the test vessels during the 48 hour exposure period. Measured concentrations were 0, 0.16, 0.44, 0.62, 1.4 and 2.1 mg/L. The EC50 is > 2.1 mg/L.
Reference
Biological results
Time (hour) |
Mean measured concentration (mg/L) |
Number immobilised per replicate |
Total number tested |
Total number immobilised |
Percentage immobilised (%) |
|||
A |
B |
C |
D |
|||||
24 |
Control |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
Solvent control |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
0.16 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
0.44 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
0.62 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
1.4 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
2.1 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
48 |
Control |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
Solvent control |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
0.16 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
0.44 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
0.62 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
1.4 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
2.1 |
0 |
0 |
0 |
0 |
20 |
0 |
0 |
|
Analytical results
Nominal concentration (mg/L) |
Measured concentration of (µg/L) |
Mean measured concentration (mg/L) |
Mean measured concentration (%) |
|||
0 hour |
48 hour |
|||||
(mg/L) |
% of nominal |
(mg/L) |
% of nominal |
|||
Control |
<LOQ |
- |
<LOQ |
- |
<LOQ |
- |
Solvent control |
<LOQ |
- |
<LOQ |
- |
<LOQ |
- |
0.3125 |
0.11 |
35 |
0.20a |
65 |
0.16 |
50 |
0.625 |
0.55 |
87 |
0.34b |
55 |
0.44 |
71 |
1.25 |
0.85 |
68 |
0.39c |
31 |
0.62 |
50 |
2.5 |
1.8 |
70 |
1.0d |
40 |
1.4 |
55 |
5.0 |
2.6 |
52 |
1.7e |
33 |
2.1 |
43 |
All measured concentrations quoted to 2 significant figures
a Mean of triplicate analyses: 0.61, <LOQ and <LOQ mg/L
b Mean of triplicate analyses: 0.35, 0.18 and 0.50 mg/L
c Mean of triplicate analyses: 0.40, 0.27 and 0.52 mg/L
d Mean of triplicate analyses: 1.0, 1.1 and 1.0 mg/L
e Mean of triplicate analyses: 1.7, 1.4 and 1.9 mg/L
The limit ofquantification (LOQ)in the study was 0.075 mg/L; <LOQ values treated as 0 when calculating means.
Description of key information
In a test according to OECD 202, 20 daphnids/concentration were exposed to 0, 0.3125, 0.625, 1.25, 2.5 and 5.0 mg/L of the substance. In the test solutions 0.1 mg/L of the solvent THF was present. No immobility was observed in any of the test vessels during the 48 hour exposure period. Measured concentrations were 0, 0.16, 0.44, 0.62, 1.4 and 2.1 mg/L. The EC50 is > 2.1 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.1 mg/L
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