Registration Dossier
Registration Dossier
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EC number: 430-960-1 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Toxicity to aquatic algae has been assessed with one substances that would fall within the TDI-II category definition. The study used water accommodated fractions as the test item is a poorly soluble UVCB. The study reported EC50 values >100 mg/L and a NOEC of 100 mg/L.
Based on these results, members of the category are not considered to be toxic to aquatic algae at their limit of solubility.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
A study was performed to assess the effects of Reaction mass of aniline and m-tolylidene diisocyanate on the growth of the green alga Pseudokirchneriella subcapitata (Vryenhoef 2018). The method followed was designed to be compatible with the OECD guideline 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test". Following a preliminary range-finding test, Pseudokirchneriella subcapitata was exposed to Water Accommodated Fractions (WAF) at a single nominal loading rate of 100 mg/L (six replicate flasks) for 96 hours, under constant illumination and shaking at a temperature of 24 ± 1°C. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter® Multisizer Particle Counter.
EL50 values for growth rate and yield were determined to be >100 mg/L WAF. Correspondingly the No Observed Effect Loading Rate was 100 mg/L WAF. It was considered unnecessary and unrealistic to test at loading rates in excess of 100 mg/L. Test concentrations at 0 and 96 hours were analysed by HPLC and determined to be 0.077 mg/L at 0 hours and 0.047 mg/L at 96 hours. Given that the toxicity cannot be attributed to a single component or a mixture of components but to the test item as a whole the results were based on nominal loading rates only.
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