Pyrogallol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: AFNOR NF T 90-301 (1974)

Version / remarks:

TESTING OF WATER - DETERMINATION OF THE INHIBITION OF THE MOBILITYOF DAPHNIA MAGNA

Deviations:

no

GLP compliance:

not specified

Vehicle:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water Flea
- Strain/clone: magna
- Justification for species other than prescribed by test guideline: not specified
- Source: not specified
- Age of parental stock (mean and range, SD): < 72h
- Feeding during test: yes
- Food type: The daphnia are fed with Chlorella vulgaris. The culture of these algae is carried out in a modified Lefevre-Czarda by the incorporation of trace elements (AFNOR T 90-304, 1980). Algal solutions were not
directly used: They were filtered on pleated filter paper PRAT-DUMAS & Co n ° 2.
- Amount: 106 chlorella / daphnia / hour
- Frequency: Food is brought daily

ACCLIMATION
- Acclimation period: not specified
- Acclimation conditions (same as test or not): not specified
- Type and amount of food: not specified
- Feeding frequency: not specified
- Health during acclimation (any mortality observed): not specified

QUARANTINE (wild caught)
- Duration: not specified
- Health/mortality: not specified

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: not specified

Daphnia breeding was made in a thermostatically controlled room at 22°C ± 0.5, photoperiod: 16 hours of iight and 8 hours of night. For reproduction of these crustaceans Cladoceres, three bins are
used (L = 30 cm, I = 16 cm, H = 20 cm). Everyone receives 4 liters of Volvic brand water and 100 daphnids of less than 72 hours. A two-day lag in getting started each tray allows to spread the production. This one started from 5 to 6 days after seeding. The first three generations were eliminated each time (Standard AFNOR T 90-301, 1974).
The bins were filtered every 3 days. When daphnids are in their optimal phase of reproduction (8 to 10 daphnia produced per mother every 2 to 3 days), the filtration is done through 3 screens whose
meshes are respectively 1600 μm, 800 μm and 560 μm. The first retains the good breeders, the second those which must be eliminated, and the third collects the daphnia less than 72 hours usable for testing. The bins
are renewed every week.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Remarks on exposure duration:

APPLICATION FREQ.: 1 X

Hardness:

not specified

Test temperature:

22 °C

pH:

pH 7-7.8

Dissolved oxygen:

A slight bubbling allows an availability of a sufficient quantity of oxygen

Salinity:

not specified

Conductivity:

160-180 umhos/cm

Nominal and measured concentrations:

10000-35000 ug/L

Details on test conditions:

The methodology used for carrying out the tests follows that recommended for preliminary tests of standards AFNOR T 90-301 (1974) and T 90-303 (1978)

The were carried out using a series of15 x 160 mm glass tubes corresponding to the different tested concentrations. Each tube received five daphnias less than 72 hours for a final volume of solution of 10 ml. The tubes are then plugged with plugs and placed in the dark in a thermostatically controlled room at 22°c ± 0.5. At different reading times, the mobility of crustaceans was observed and reported as well as the different manifestations of their locomotor behaviors. The concentrations are defined which prevent the daphnids from moving normally within fifteen seconds after a slight stirring of the tubes (Cl). The Authors considered as immobilized, the daphnies that only moved their antennas or which turned on themselves (phenomena observed with poorly soluble products like endosulfan, the endine ...).

Reference substance (positive control):

not required

Duration:

2 h

Dose descriptor:

EC50

Effect conc.:

> 100 000 µg/L

Nominal / measured:

not specified

Conc. based on:

act. ingr.

Basis for effect:

other: intoxication, immobilization

Remarks:

Effective concentration to 50% of test organisms

Duration:

4 h

Dose descriptor:

EC50

Effect conc.:

> 100 000 µg/L

Nominal / measured:

not specified

Conc. based on:

act. ingr.

Basis for effect:

other: intoxication, immobilization

Remarks:

Effective concentration to 50% of test organisms

Duration:

6 h

Dose descriptor:

EC50

Effect conc.:

> 35 000 - < 100 000 µg/L

Nominal / measured:

not specified

Conc. based on:

act. ingr.

Basis for effect:

other: intoxication, immobilization

Remarks:

Effective concentration to 50% of test organisms

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 10 000 - < 35 000 µg/L

Nominal / measured:

not specified

Conc. based on:

act. ingr.

Basis for effect:

other: intoxication, immobilization

Remarks:

Effective concentration to 50% of test organisms

Details on results:

refer to the table of results

Results with reference substance (positive control):

not required

Reported statistics and error estimates:

not specified

CI 50 2h "a vue"	CI 50 4h  "a vue"	CI 50 6h "a vue"	CI 50 24h "a vue"
> 100 mg/l	> 100 mg/l	35 - 100 mg/l	10 - 35 mg/l

Validity criteria fulfilled:

yes

Conclusions:

The IC 50 (24h) was in the range 10 - 35 mg/l  for Daphnia Magna, at these test conditions.
Considering the results, accoring to CLP classification critiria, the substance was not classifiedas hazardous to the aquatic environment (short-term).

Executive summary:

The acute toxicity of analytical grade of Pyrogallol has been tested on Daphnia Magna Stratus after different periods of contact: 2, 4, 6 and 24 hours. The IC50 were determined.

Description of key information

The acute toxicity of Pyrogallol was tested on Daphnia magna and observed at 2, 4, 6 and 24 hours, according to

IS0/6341; the preliminary test was performed according to AFNOR T 90-301 (1974)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

10 mg/L

Additional information

The EC 50 (24h) was in the range 10 - 35 mg/l  for Daphnia Magna, at these test conditions.

Considering the results, accoring to CLP classification critiria, the substance was not classifiedas hazardous to the aquatic environment (short-term).