Chlorbutanol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15-Sep-2017 to 09-Mar-2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,1,1-trichloro-2-methyl-2-propanol hemihydrate

Cas Number:

6001-64-5

Molecular formula:

C4H7Cl3O H2O

IUPAC Name:

1,1,1-trichloro-2-methyl-2-propanol hemihydrate

Test material form:

solid: crystalline

Details on test material:

transparent and crystalline solid at ambient temperature

Sampling and analysis

Analytical monitoring:

yes

Remarks:

GC-MS

Details on sampling:

Specimens were drawn from freshly prepared treatments and those incubated for 48 hrs. The supporting analysis of the specimens could not be performed on the same day of collection of the specimens. Therefore, all specimens from freshly prepared test solutions and those incubated for 48 hrs were stored deep-frozen at <= -18°C in glass bottles and analysed after thawing.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS (clone 5) of SGS INSTITUT FRESENIUS Iabaratory bred (derived from Dr. M. Bergtold, BASF, D-67117 Limburgerhof) was used for the test. The cultivation of the daphnia is performed in a way that the animals are transferred in new test medium at an interval of 1 to 3 days followed by feeding with Desmodesmus subspicatus- and/or Ch/ore//a-cells. Daphnia used for the test are not older than 24hr. Therefore, in every case, daphnias are transferred into fresh medium one day before starting of the daphnia test in order to get daphnia for the test younger than 24hr. During the test the daphnia were not fed.

Study design

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

20 ± 1°C

pH:

8.2 ± 0.4

Dissolved oxygen:

8.2 ± 0.4 mg/L

Nominal and measured concentrations:

0, 40, 60, 90, 135 and 202.5 mg/L
As a result of the supporting analyse it can be stated that the results of the biological part should be based on the nominal concentrations applied because the necessary stability of ~ 80% could be shown.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 d

Dose descriptor:

EC10

Effect conc.:

74.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

110.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

ln order to verify the sensitivity of the test system towards toxic items the effect of potassium dichromate towards different animals of this breed was tested prior to beginning of the test. The value of the EC50 (24h) in this period of time was at 0.97 mg/L potassium dichromate. This value is in accordance with the recommended range given in OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test) being 0.6 to 2.1 mg/L potassium dichromate given in OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test) and the range given in the study plan.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test is considered valid as all conditions for validity were met.

Executive summary:

Chlorobutanol Hemihydrate was tested for acute toxicity towards daphnia according to OECD Test Guideline 202 (version dated 13-Apr-2004). ln order to investigate the influence of the test item towards the daphnia, the swimming behaviour of the animals was recorded. Under the conditions used for the test, there was an immobilisation of the daphnia at concentrations >60 mg/L. As a conclusion of the analytical part of this study, it can be stated that the concentrations Chlorobutanol Hemihydrate remained sufficiently stable during incubation of 48hrs. As a result of the supporting analyse it can be stated that the results of the biological part should be based on the nominal concentrations applied because the recoveries of the nominal concentrations were all >80%.