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EC number: 825-814-0 | CAS number: 222721-94-0
The source substance Tri (hexyl, octyl, decyl) is not toxic in the range of water solubility. Based on Read-across (please see Analogue Approach) it is expected that the same is valid for 1,2,3-Propanetricarboxylic acid, 2-hydroxy-, tri-C14-15-alkyl esters.
Due to low water solubility limit of the test substance a filtrate of a supersaturated stock suspension of nominal 100 mg/L was tested. Thus no concentrations above the solubility limit of the test substance in the used test water were tested. Due to the low water solubility limit no analytical dose verification could be done in the present test. Therefore, the biological results could not be related to a specific concentration but to the solubility limit of Tri (hexyl, octyl, decyl) citrate in the test medium.
In the control and in the undiluted filtrate of the supersaturated stock suspension all fishes survived until the end of the test and no signs of intoxication were observed. Therefore, the 96-hour NOEC and the 96-hour LC0 of Tri (hexyl, octyl, decyl) citrate to Zebra fish were determined to be at least up to the solubility limit. The NOEC and the LC0 might even be higher, but concentrations in excess of the solubility limit had not been tested. The 96-hour LOEC, the 96-hour LC50 and the 96-hour LC100 were clearly higher than the solubility limit of the test substance in the used test water.
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