Reaction mass of N-[3-(dimethylamino)propyl]docosanamide and N-[3-(dimethylamino)propyl]stearamide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01/2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of glycerol and N-[3-(dimethylamino)propyl]stearamide and N-[3-(dimethylamino)propyl]docosanamide

Molecular formula:

C21 H50 N O C23 H54 N O C25 H58 N O C27 H62 N O

IUPAC Name:

Reaction mass of glycerol and N-[3-(dimethylamino)propyl]stearamide and N-[3-(dimethylamino)propyl]docosanamide

Test material form:

solid: flakes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Name of Species : Daphnia magna
No. of Daphnia group : 20 Daphnids per concentration (four replicates of single
Concentration and each containing Five Daphnids)
Temperature : 18 - 22°C
No. of Dose concentration : 5

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Details on test conditions:

Temperature : 21.2-21.6 °C
Light conditions : 16 hours light and 8 hours dark cycle
Immobilization : In the control not more than 10 % of the Daphnia should immobilize
during the test period.
Test concentration : A limit test was performed at a dose concentration of 100 mg/l of the test
item prior to the main study. On the basis of the findings main study was
conducted by preparing five concentrations of the test item (i.e. 2, 4, 8,
10, 20 mg/l).
Carrier solvent used : Carboxymethyl Cellulose (at 30 mg/l concentration)
Water used : Ground Water
pH of water : 6.0-7.2
Dissolved oxygen : ≥ 4 mg/l content of the water

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

48.75 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

EC50 : 48.75 mg/l of water
95 % F. limit : 40.23 - 57.17
Lowest concentration causing 100 % immobility in Daphnia : 100 mg/ l of water

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this study i.e. EC50 of Daphnia with “Brassicamidopropyl Dimethylamine”
came out as:
EC50 : 48.75 mg/l of water
95 % F. limit : 40.23 - 57.17
The test item has been tested as per ‘OECD Guideline for the testing of Chemicals, Daphnia Acute
Immobilization Test Guideline No. 202 adopted on 13th April 2004’

Executive summary:

The study was conducted to determine the ‘Immobilization test of ‘Daphnia magna’ (a benthic

zooplankton strain) with ‘Brassicamidopropyl Dimethylamine.

In the present study, a limit test was performed at 100 mg/l concentration, of the test item

‘Brassicamidopropyl Dimethylamine’ with 20 Daphnia (four replicates of five Daphnids each).

Based on the result of the limit test, the Main Test was conducted taking five different

concentrations of the test item (20, 40, 60, 80 and 100 mg/l). Similarly 20 Daphnia (four

replicates of five Daphnids each) for negative control (water) and solvent control (at 30mg/l

concentration of CMC) groups were used (the volume of solvent, used in the test concentrations

and control was kept same).

On the basis of these concentrations, the EC50 was estimated, which came out as:

EC50 : 48.75mg/l of water

95 % F. limit : 40.23 - 57.17

Lowest concentration causing 100 % immobility in Daphnia : 100 mg/ l of water