Registration Dossier
Registration Dossier
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EC number: 237-340-6 | CAS number: 13755-29-8
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes
Test material
- Reference substance name:
- Sodium tetrafluoroborate
- EC Number:
- 237-340-6
- EC Name:
- Sodium tetrafluoroborate
- Cas Number:
- 13755-29-8
- Molecular formula:
- NaBF4
- IUPAC Name:
- sodium tetrafluoroborate
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- The study will be performed with aerobic activated sewage sludge from the aeration stage of a local wastewater treatment plant, ARA Birs (Birsfelden / Switzerland), which treats predominantly domestic sewage
Study design
- Water media type:
- other: Deionized water, containing less than 1 mg/L DOC (dissolved organic carbon)
Test conditions
- Test temperature:
- room temperature
- pH:
- 7.2
- Dissolved oxygen:
- less than 1 mg/L DOC
- Nominal and measured concentrations:
- 563, 750 and 100 mg/L, with a spacing factor of 1.33 and with five replicates per concentration. The selection of the test concentrations and replicates number aimed to primarily permit the determination of a NOEC.
As a positive control, the reference item 3,5 dichlorophenol was tested under identical test conditions, at concentrations of 3.2, 10 and 32 mg/L (all single replicates).
Six blank controls, containing an equal volume of activated sludge and synthetic medium but no test or reference item, were included in the definitive test. - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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