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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Title:
Unnamed
Year:
1976

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
no
Remarks:
Not applicable ( prior to 1981)
Type of assay:
in vitro mammalian cell transformation assay

Test material

Constituent 1
Reference substance name:
Reaction mass of phosphonic acid, methyl-, bis[(5-ethyl-2-methyl-2,2-dioxido-1,3,2-dioxaphosphorinan-5-yl)methyl] ester with (5-ethyl-2-methyl-2-oxido-1,3,2-dioxaphosphorinan-5-yl)methyl methyl methylphosphonate
EC Number:
915-680-2
Molecular formula:
not applicable for UVCB
IUPAC Name:
Reaction mass of phosphonic acid, methyl-, bis[(5-ethyl-2-methyl-2,2-dioxido-1,3,2-dioxaphosphorinan-5-yl)methyl] ester with (5-ethyl-2-methyl-2-oxido-1,3,2-dioxaphosphorinan-5-yl)methyl methyl methylphosphonate

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Details on mammalian cell type (if applicable):
different strains of indicator microorganisms ( S cerevisiae (strain D4), Salmonella typhimurium strains TA 1535, TA1537, TA1538, TA-98 TA-100).
Metabolic activation:
with and without
Test concentrations with justification for top dose:
The dose range employed was 0.01 μL to 5μL per plate.
Vehicle / solvent:
DMSO
Controls
Positive controls:
yes
Positive control substance:
not specified
Details on test system and experimental conditions:
The results of the tests conducted in the absence of a metabolic system were all negative
The results of the tests conducted in the presence of the rat liver activation system were all negative

Results and discussion

Test results
Key result
Species / strain:
other: indicator microorganisms ( S cerevisiae (strain D4), Salmonella typhimurium strains TA 1535, TA1537, TA1538, TA-98 TA-100).
Metabolic activation:
not specified
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
valid
Positive controls validity:
valid

Applicant's summary and conclusion

Conclusions:
The results of the tests conducted in the absence of a metabolic system were all negative
The results of the tests conducted in the presence of the rat liver activation system were all negative. The substance did not demonstrate mutagenic activity in any of the assays conducted in this evaluation and is considered not mutagenic under these test conditions.
Executive summary:

The results of the tests conducted in the absence of a metabolic system were all negative

The results of the tests conducted in the presence of the rat liver activation system were all negative. The substance did not demonstrate mutagenic activity in any of the assays conducted in this evaluation and is considered not mutagenic under these test conditions.