Fatty acids, C16, C18 and C18-unsaturated, C12-15 alcohol (linear and branched), esters

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because the substance is highly insoluble in water, hence indicating that aquatic toxicity is unlikely to occur

the study does not need to be conducted because a long-term aquatic toxicity study on invertebrates is available

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

04 Mar 2009

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

other: Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, OECD 23

GLP compliance:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test solutions were prepared according to OECD 23. Aqueous solutions were saturated with the test item by gentle magnetic stirring for 68 h (30 - 60 rpm), followed by 4 h of phase separation. Each aqueous phase, containing the soluble substance (Water Accommodated Fraction) was used for the test.
- Differential loading: Yes
- Controls: Blank without test item

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Source: BfB Oil research Laboratory
- Breeding method: Acyclic parthenogenesis from at least the third generation (start: 17 Feb 2009)
- Age of daphnia at test start: < 24 h old

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 ± 1 °C

pH:

Test end: 7.8 (control), 7.6 (lowest exposure concentration where all daphnids are immobilized)

Dissolved oxygen:

7.7 mg O2/L (dilution water)
> 3 mg O2/L at the end of the test

Nominal and measured concentrations:

0.142, 0.295, 0.750, 1.54, and 3.02 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- No. of organisms per batch: 20
- Blank: Two tubes with 10 daphnids each

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to OECD guideline 202

OTHER TEST CONDITIONS
- Photoperiod: Darkness

EFFECT PARAMETERS MEASURED:
- Mobility: after 48 h

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 1 g/L

Nominal / measured:

nominal

Conc. based on:

other:

Remarks:

WAF

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: No

Reported statistics and error estimates:

Regression analysis

RESULTS

The lowest concentration corresponding to 100% immobilization after 48 h is > 3.00 g/1000 g*.

The highest concentration corresponding to 0% immobilization after 48 h is < 0 .150 g/1000 g*.

* Loading ratio (mass ratio of test substance and test medium in the diphasic reactor) while operating the "WAF"

Validity criteria fulfilled:

not specified

Description of key information

Data waiving: a long-term aquatic study on invertebrates is available.