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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989-04-24 to 1989-04-26
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: The strain of Daphnia magna used was obtained from Institut National de Recherche Chimique Appliquee, France, and bred since the beginning of 1978 in the Labor für Umweltanalytik und Ökologie of the BASF Ludwigshafen.
- Age of parental stock: 2 -24 h



Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.89 mmol/L
Test temperature:
18.85 to 20.85
pH:
8.1
Conductivity:
600 - 700 µS/cm
Nominal and measured concentrations:
0, 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078, 0.039 and 0.019 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel, test volume: Reagent tubes with fiat bottom, 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The synthetic medium M4 is used for the culture and test. The medium is prepared an the basis of an ultrapure, deionized water (conductivity < 0.05 µS/cm)

EFFECT PARAMETERS MEASURED: At the beginning and after 3, 6, 24, 48 h Daphnia magna's inability to swim is recorded. Those animals which are unable to swim after gentle agitation of the test vessels are regarded as unable to swim.



Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CI: 0.12 - 0.2 mg/L

Table 1: Animals able to swim

Conc. [mg/L]

Animals able to swim after

0 h

3 h

6 h

24 h

48 h

0.019

20

20

20

20

20

0.039

20

20

20

20

19

0.078

20

20

20

20

18

0.156

20

20

20

19

9

0.312

20

20

20

17

4

0.625

20

20

20

4

0

1.25

20

20

20

1

0

2.5

20

20

20

0

0

5

20

20

19

0

0

10

20

20

2

0

0

0

20

20

20

20

20

 

Conclusions:
Under the conditions of this acute daphnia test an EC50 of 0.16 mg/L (95% CI: 0.12 - 0.2 mg/L) was determined for the test item.
Executive summary:

The acute toxicity of the test substance to Daphnia magna was determined in a 48-hour static test conducted equivalent to OECD guideline 202. The daphnids were exposed to the following nominal test item concentrations: 0, 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078, 0.039 and 0.019 mg/L. Under the conditions of this test, an EC50 of 0.16 mg/L (95% CI: 0.12 - 0.2 mg/L) was determined.

Description of key information

In an acute toxicity test with Daphnia magna conducted equivalent to OECD guideline 202 an 48h-EC50 of 0.16 mg/L was determined for the test item.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.16 mg/L

Additional information

The acute toxicity of the test substance to Daphnia magna was determined in a 48-hour static test conducted equivalent to OECD guideline 202. The daphnids were exposed to the following nominal test item concentrations: 0, 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078, 0.039 and 0.019 mg/L. Under the conditions of this test, an EC50 of 0.16 mg/L (95% CI: 0.12 - 0.2 mg/L) was determined.