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EC number: 306-238-4 | CAS number: 96690-44-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From 04 April, 2012 to 26 April, 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- KL2 due to RA
- Justification for type of information:
- Refer to section 13 for details on the read-across justification. The study with the read across substance is considered sufficient to fulfil the information requirements as further explained in the provided endpoint summary.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- C12-14 alkyl ethyl dimethyl ethyl sulphates QAS was added to the incubation vessels using a stock solution of approximately 1.0 g/L.The stock solution of quaternary ammonium compounds, C12-14 (evennumbered)-alkylethyldimethyl, ethyl sulphates was prepared by dissolving 0.78 g C12-14 alkyl ethyl dimethyl ethyl sulphates QAS in 780 mL deionized water.
pH stock solution C12-14 alkyl ethyl dimethyl ethyl sulphates QAS =6.6 - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Unadapted secondary activated sludge was obtained (24-04-2012) from the WWTP Nieuwgraaf in Duiven. The WWTP Duiven is an activated sludge plant treating predominantly domestic wastewater. Prior to use the activated sludge was homogenized with a syringe. The dry weight of the homogenized activated sludge was determined and subsequently concentrated by settlement to the required dry weight concentration. The dry weight of the activated sludge in the incubation vessels was 1.5 g/L.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- Temperature varied from 19.8 to 20.6°C.
- pH:
- The pH of the reaction mixtures after the incubation period ranged from 7.2 to 7.4.
- Dissolved oxygen:
- aerobic during test
- Details on test conditions:
- -The test was performed in 300 mL Erlenmeyers.
-The homogenized activated sludge was incubated in a shaking water bath (100 rpm, 20°C) for 3 hours with various concentrations of the test
compound and synthetic sewage.
-Synthetic sewage was added to the activated sludge (1.6 mL on a total volume of 50 mL).
Synthetic sewage contained per liter: 16 g peptone
11 g meat extract
3 g urea
0.7 g NaCl
0.3 g CaCl2
0.2 g MgSO4.7H2O
2.8 g K2HPO4
-Different concentrations (spaced by a factor two) of test compound and reference compound where added using stock solutions both of 1.0 g/L
-Tap-water was used to fill up the total test volume to 50 mlL
- The dry weight concentration of the activated sludge in the test was 1.5 g/L.
-The tested concentrations of the reference compound 3,5-dichlorophenol where: 2.5 mg/L, 5.0 mg/L,10.0 mg/L, 20.0 mg/L and 40.0 mg/L
-The tested concentrations of hydrogenated (tallow alkyl) trimethyl ammonium chloride where : 10 mg/L, 20 mg/L, 40 mg/L, 80 mg/L and 160 mg/L.
-Controls without test substance (or reference compound) are measured at the beginning and at the end of the test
-The inhibitory effect of the test substance/reference compound is expressed as a percentage of the mean of the two control respiration rates.
-An abiotic control was not performed because neither reduction of oxygen by the test substance nor formation of oxygen due to chemical
breakdown of the test substance is expected.
-No specific analyses were performed - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Test conditions
The pH of the reaction mixtures after the incubation period ranged from 7.2 to 7.4. Temperature varied from 19.8 to 20.6°C. These conditions allow
respiration of the activated sludge used.
Validity of the test
The validity of the test is demonstrated by three criteria. First, the coefficient of variation of the replicates of the control oxygen uptake rates was 10%, which fulfils the maximum prescribed variation of <30%. Secondly, the prescribed average oxygen uptake rate of > 20 mg O2/g dry weight/hour for the control measurements was achieved (30 mg O2/g dry weight/hour). The third criterion was met as shown by the EC50 of the reference compound of 15 mg/L, which is within the prescribed range of 2 to 25 mg/L.
Activated sludge respiration inhibition test
The inhibitory effect of quaternary ammonium compounds, C12-14 (even-numbered)-alkylethyldimethyl, ethyl sulphates at a particular concentration is expressed as a percentage of the two controls. From the results EC values were calculated.
The EC50 of quaternary ammonium compounds, C12- 14 (even-numbered)-alkylethyldimethyl, ethyl sulphates for activated sludge after 3 hours contact time is 30 mg/L with 95% confidence limits of 13 and 72 mg/L. The EC10, EC20 and EC80 are 9, 14 and 66 mg/L, respectively. - Results with reference substance (positive control):
- The EC50 of the reference compound was 15 mg/L at a contact time of 3 h, respectively. These EC50 values is within the prescribed range of 2 to 25 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, 3 h EC50 and EC10 values of test substance in sludge after 3 h of contact time was determined to be 30 and 9 mg/L (nominal) respectively.
- Executive summary:
A study was conducted to determine the toxicity of the read-across substance C12 -14 ADMA ES to microorganisms in an activated sludge respiration inhibition testaccording to OECD Guideline 209, in compliance with GLP. The test was valid as shown by the EC50 of the reference substance 3,5 -dichlorophenol (tested at 15 mg/L), the coefficient of variation for oxygen uptake rates in control (10%) and the average oxygen uptake rate for the control replicates (30 mg O2/g dw/h). The toxicity of the test substance to activated sludge was determined for an exposure period of 3 h, using various concentrations of the test substance. The inhibitory effect of the substance at a particular concentration is expressed as a percentage of the two controls. No analytical determination was performed for the test substance. Under the study conditions, 3 h EC50 of the test substance in sludge was determined to be 30 mg/L (95% C.I. 13 - 72 mg/L). The 3 h EC10 was determined to be 9 mg/L (Geerts, 2012).
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 30 mg/L
- EC10 or NOEC for microorganisms:
- 9 mg/L
Additional information
A study was conducted to determine the toxicity of the read-across substance C12 -14 ADMA ES to microorganisms in an activated sludge respiration inhibition testaccording to OECD Guideline 209, in compliance with GLP. The test was valid as shown by the EC50 of the reference substance 3,5 -dichlorophenol (tested at 15 mg/L), the coefficient of variation for oxygen uptake rates in control (10%) and the average oxygen uptake rate for the control replicates (30 mg O2/g dw/h). The toxicity of the test substance to activated sludge was determined for an exposure period of 3 h, using various concentrations of the test substance. The inhibitory effect of the substance at a particular concentration is expressed as a percentage of the two controls. No analytical determination was performed for the test substance. Under the study conditions, 3 h EC50 of the test substance in sludge was determined to be 30 mg/L (95% C.I. 13 - 72 mg/L). The 3 h EC10 was determined to be 9 mg/L (Geerts, 2012).
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