Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
other: The test follows the EU Directive 79/831/EEC, Annex V, part C
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: test solution was added without any pretreatment.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain: Straus
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 hours
- Feeding during test
- Food type: Whilst changing water Brewer's yeast was given, also 1 x daily grown Green algae culture


Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.70 +/- 0.5 mmol/L
Test temperature:
18-20 °C
pH:
about 8.0
Dissolved oxygen:
7.1 - 8.6 mg/L
Nominal and measured concentrations:
The test medium of the highest test concentration, 125 mg/L (nominal) was prepared. Subsequently, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations. A control was tested in parallel.
Nominal concentrations: 7.81, 15.6, 31.2, 62.5, 125 mg/mL
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom glass tubes
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the test guideline DIN 38412, L 11
- Culture medium different from test medium: Not specified

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: diffuse light (5 microeinstein/m*m*s) at 400 - 750 nm wave length

EFFECT PARAMETERS MEASURED: mobility; visually after 0, 3, 6, 24, 48 h
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
31.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
42.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
62.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
The EC50 (24-h and 48-h) was determined by the moving avrage method. The EC0 was determined by the highest concentration at which <= 10% immobilisation occurred. The EC0 was determined by the lowest concentration at which 100 % immobilisation occurred.

Table: Mobile daphnids after 0, 24 and 48 hours of exposure

Concentration [mg/L] Mobile test animals after 
0 h 24 h 48 h
7.01 20 20 20
15.6 20 20 20
31.2 20 20 19
62.5 20 5 0
125 20 0 0
0 20 20 20
Validity criteria fulfilled:
not specified
Conclusions:
In an aquatic test with Daphnia magna according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202 the 48-hour EC50 of the test item was determined to be 42.7 mg/L (nominal).
Executive summary:

The toxicity of the test item to aquatic invertebrates was assessed in a static, 48-hour test according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202. Young daphnids (2 - 24 hours old; 5 animals per test vessel) were exposed to nominal test item concentrations of: 7.81, 15.6, 31.2, 62.5, 125 mg/mL (4 replicates per concentration). A negative control (dilution water only; five replicates) was tested in parallel. The solution water used for the test was prepared according to the test guideline DIN 38412, L 11. Daphnids were fed during the test with Brewer's yeast and green algae (daily). The light regime was 16 light : 8 darkness at a test temperature of 18-20 °C.  Mobility of the test animals was checked visually after 0, 3, 6, 24 and 48 hours of exposure. In result, the 48-hour EC0 and EC100 of the test item was determined to be 31.2 mg/L and 62.5 mg/L in nominal, respectively. The 48-hour EC50 of the test item was 42.7 mg/L (nominal).

Description of key information

In a test with Daphnia magna according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202, the 48-hour EC50 of the test item was determined to be 42.7 mg/L (nominal).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
42.7 mg/L

Additional information

The toxicity of the test item to aquatic invertebrates was assessed in a static, 48-hour test according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202. Young daphnids (2 - 24 hours old; 5 animals per test vessel) were exposed to nominal test item concentrations of: 7.81, 15.6, 31.2, 62.5, 125 mg/mL (4 replicates per concentration). A negative control (dilution water only; five replicates) was tested in parallel. The solution water used for the test was prepared according to the test guideline DIN 38412, L 11. Daphnids were fed during the test with Brewer's yeast and green algae (daily). The light regime was 16 light : 8 darkness at a test temperature of 18-20 °C.  Mobility of the test animals was checked visually after 0, 3, 6, 24 and 48 hours of exposure. In result, the 48-hour EC0 and EC100 of the test item was determined to be 31.2 mg/L and 62.5 mg/L in nominal, respectively. The 48-hour EC50 of the test item was 42.7 mg/L (nominal).