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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:
biodegradation in water: simulation testing on ultimate degradation in surface water
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20 days, August, 2011
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Standard sea-water study required for use of chemical substances in off-shore applications.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report Date:
2011

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 306 (Biodegradability in Seawater)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Radiolabelling:
no

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
natural water: marine
Details on source and properties of surface water:
Seawater was buffered with 1 mL per liter of pH 7.2 Phosphate Buffer Solution. Trace inorganic nutrient solutions were added in corresponding 1 mL per liter volumes. In addition, 10 mg of Hach 2533 nitrification inhibitor per liter of dilution water was added to minimize possible interference caused by the oxidation of nitrogenous compounds by potential nitrifying microorganism.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
2 mg/L
Reference substance
Reference substance:
other: sodium acetate

Results and discussion

% Degradation
Key result
% Degr.:
ca. 70
Parameter:
O2 consumption
Sampling time:
20 d
Transformation products:
not measured

Any other information on results incl. tables

The chimica oxygen demand (COD) for Formaldehyde, reaction products with ethylenediamine was determined to be 0.71 mg O2 per mg substance. The maximum percent biodegradation for Formaldehyde, reaction products with ethylenediamine of 70% was observed on days 20 and 28. The sodium acetate 2 mg/L reference control achieved greater than 60% biodegradation within the 28 day test period i.e. maxium biodegradation of 103% was observed on day 13. Dissolved oxygen depletion in the seawater controls remained within the test guideline of 30%; on day 28 a maximum dissolved oxygen depletion of 6.8% occurred (control oxygen levels had decreased from 7.4 mg/L on day 0, to 6.9 mg/L on day 28).

Examination of control seawater and seawater containing 2 mg/L ofFormaldehyde, reaction products with ethylenediaminefor possible nitrite (NO2 and NO3) formation was conducted on days 27 and 28. No evidence of nitrification was evident; nitrite and nitrate levels for exposure bottles were at or below those of the seawater control at these times.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The chimica oxygen demand (COD) for Formaldehyde, reaction products with ethylenediamine was determined to be 0.71 mg O2 per mg substance. The maximum percent biodegradation for Formaldehyde, reaction products with ethylenediamine of 70% was observed on days 20 and 28.
Considered biodegradable.