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EC number: 290-611-0 | CAS number: 90194-04-0
Table 1: Dissolved Oxygen Concentrations of the Test Preparations after 30 Minutes Contact Time in the Range-Finding Test
Dissolved Oxygen Concentrations of the Test Preparations after
30 Minutes Contact Time in the Definitive Test
Dissolved Oxygen Concentration
Table 2: Oxygen Consumption Rates and Percentage Inhibition Values after 3 Hours Contact Time in the Range-Finding Test
Final O2 Reading
O2 Consumption Rates
Test Item 10
R1 – R4 = Replicates 1 to 4
[Increase in respiration rate as compared to controls]
Table 3 Dissolved Oxygen Concentrations of the Test Preparations after 30 Minutes Contact Time in the Definitive Test
R1 – R3 = Replicates 1 to 3
Table 4 Oxygen Consumption Rates and Percentage Inhibition Values after 3 Hours Contact Time in the Definitive Test
R1 – R3 = Replicates 1 to 3
A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2010) No. 209 "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)".
Following a preliminary range-finding test, activated sewage sludge was exposed to an aqueous dispersion of the test item at concentrations of 10, 32, 100, 320 and 1000 mg/L for a period of 3 hours at measured temperatures of between 21 and 22 deg C with the addition of a synthetic sewage as a respiratory substrate.
The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5-dichlorophenol.
The effect of the test item on the respiration of activated sewage sludge gave a 3 Hour EC50 value of 160 mg/L, 95% confidence limits 140 to 190 mg/L.
The reference item gave a 3-Hour EC50 value of 7.6 mg/L, 95% confidence limits 5.9 to 9.8 mg/L.
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