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EC number: 290-611-0 | CAS number: 90194-04-0
Table 2: Cumulative Immobilization Data and Observations in the Definitive Test
Nominal Loading Rate(mg/L)
Cumulative Immobilized Daphnia(Initial Population: 5 Per Replicate)
R = Replicate
N = Normal
AI =All daphnia immobilized
A study was performed to assess the acute toxicity of the test item toDaphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphniasp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.
Due to the low aqueous solubility and complex nature of the test item, for the purposes of the test, the test medium was prepared as a Water Accommodated Fraction (WAF).
Following a preliminary range‑finding test and initial experiment, 20 daphnids (4 replicates of 5 animals) were exposed to WAFs of the test item over a range of nominal loading rates of1.0,1.8,3.2,5.6mg/L and10 mg/L for 48 hours at a temperature of 21 °C to 22 °C under semi‑static test conditions. The number of immobilized daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.
Chemical analysis of the 1.0, 1.8, 3.2 and 5.6 mg/L loading rate WAF fresh test preparations at 0 and 24 hours showed measured test concentrations to range from 0.38 to 6.0 mg/L. Chemical analysis of the aged test preparations at 24 and 48 hours showed measured test concentrations to range from 0.25 to 4.4 mg/L.
The dissolved test item may have been one or several components of the test item. Given that the toxicity cannot be attributed to a single component or a mixture of components, but to the test item as a whole, the results were based on nominal loading rates only.
Exposure ofDaphnia magna to the test item gave the following results:
EL50(mg/L Loading RateWAF)
95% Confidence Limits (mg/L Loading Rate WAF)
NOEL = No Observed Effect Loading rate
LOEL = Lowest Observed Effect Loading rate
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