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Biodegradation in water and sediment: simulation tests

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Endpoint:
biodegradation in water: sewage treatment simulation testing
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)
GLP compliance:
no
Radiolabelling:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial, adapted
Duration of test (contact time):
8 wk
Initial conc.:
5 mg/L
Based on:
act. ingr.
Initial conc.:
10 mg/L
Based on:
act. ingr.
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
- 2 weeks adaptation phase: 2 units with 1 and 2 mg a.i./L
- afterwards the laboratory units were run for 8 weeks
% Degr.:
98
St. dev.:
0.8
Parameter:
test mat. analysis
Sampling time:
18 d
Remarks on result:
other: mean daily degradation rate measured from day 18 to day 39
Transformation products:
not measured

 

5 mg a.i./L

 

 

10 mg a.i./L

 

 

Adaptation phase

mg a.i./L ininflow

mg a.i./L ineffluent

% degradation

mg a.i./L ininflow

mg a.i./L ineffluent

% degradation

1

5.2

1.4

73

11.0

3.9

65

2

6.1

0.9

85

9.2

2.9

68

3

6.3

2.3

63

8.9

2.8

69

5

5.0

1.0

80

10.1

2.5

75

8

5.1

1.2

76

10.0

2.2

78

12

6.0

0.7

88

11.0

0.8

93

13

6.8

0.9

87

10.1

1.7

83

14

5.2

0.6

88

9.8

1.6

84

15

4.9

0.5

90

10.0

1.6

84

16

5.7

0.5

91

11.0

1.4

87

17

5.3

0.5

91

12.0

1.0

92

Evaluation phase

 

 

 

 

 

 

18

5.2

0.2

96

9.8

0.5

95

19

5.4

0.2

96

9.8

0.3

97

20

4.9

0.2

96

10.1

0.3

97

22

5.5

0.1

98

10.2

0.3

97

24

6.0

0.2

97

10.3

0.1

99

26

5.8

0.2

97

11.0

0.1

99

28

5.0

0.1

98

9.5

0.1

99

29

4.9

0.1

98

9.8

0.2

98

31

5.1

0.1

98

10.0

0.1

99

33

5.3

0.1

98

10.1

0

100

34

5.2

0.1

98

9.5

0.1

99

35

5.0

0

100

11.2

0.1

99

36

6.1

0

100

10.2

0

100

37

4.9

0

100

10.0

0.1

99

39

5.2

0

100

9.2

0

100

Validity criteria fulfilled:
not specified
Conclusions:
In an OECD confirmatory test, the degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid (5 and 10 mg a.i./L using industrial adapted sludge) was determined to be 98%.
Executive summary:

The degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid was studied in an OECD confirmatory test.

Two model activated sludge units with industrial sludge were run in parallel. After an adaptation phase of 2 weeks (1 and 2 mg a.i./L) the concentration of the test item was increased to 10 or 20 mg a.i./L, respectively. The units were run for 8 weeks.

The degradation of the test item was determined using the Orange II method (Few, A. V., Ottewill, R. H.: J. Colloid. Sci, 11 (1956) 34; Scott, G. V.: Anal. Chem., 40 (1968) 768).

The mean degradation was determined based on daily relative elimination of the test item starting after the adaptation phase for 21 consecutive days. The mean daily degradation rate was 98%.

Endpoint:
biodegradation in water: sewage treatment simulation testing
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)
GLP compliance:
no
Radiolabelling:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial, adapted
Duration of test (contact time):
8 wk
Initial conc.:
10 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
- 2 weeks adaptation phase: 2 units with 2 and 5 mg DOC/L
- afterwards the laboratory units were run for 5 weeks
% Degr.:
75
St. dev.:
4.8
Parameter:
test mat. analysis
Sampling time:
18 d
Remarks on result:
other: mean daily degradation rate measured from day 18 to day 48
Transformation products:
not measured

Adaptation phase

mg DOC/L in inflow

mg DOC/L in effluent

% degradation

1

15

8.2

22.3

2

16.8

10.6

29.1

3

13.5

7.9

36

5

12.3

8.6

57.7

8

13.3

10.1

63.4

12

13.5

10.8

69.1

13

12.3

9.6

69.1

15

10.6

7.7

66.9

17

11.1

7.7

61.1

Evaluation phase

 

 

 

18

13.3

10.1

63.4

19

12.3

8.6

57.7

21

13.5

10.8

69.1

23

12

9.7

73.7

24

12.3

9.6

69.1

26

9.1

7.9

86.3

27

10.6

7.7

66.9

29

10.1

8.2

78.3

31

11.1

8.6

71.4

32

9.6

7.7

78.3

34

9.1

8.4

92

35

8.9

7.9

88.6

37

11.1

7.7

61.1

39

9.6

6.8

68

40

14

10.8

63.4

42

11.5

8.6

66.9

43

12

10.6

84

44

11.5

10.6

89.7

46

11.1

10.1

88.6

47

9.6

8.2

84

48

10.6

8.6

77.1

Validity criteria fulfilled:
not specified
Conclusions:
The degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid (10 mg DOC/L using industrial adapted sludge) was determined to be 75%.
Executive summary:

The degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid was studied in a coupled units etst according to OECD Guideline 303 A..

Two model activated sludge units with industrial sludge were run in parallel. After an adaptation phase of 2 weeks (2 and 5 mg DOC/L) the concentration of the test item was increased to 10 mg DOC/L, respectively. The units were run for 5 weeks.

The mean degradation was determined based on daily relative elimination of the test item starting after the adaptation phase for 30 consecutive days. The mean daily degradation rate was 75%.

Description of key information

degradation rate 75% (ultimate degradation) after adaptation

Key value for chemical safety assessment

Additional information

The degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid was studied in a coupled units test according to OECD Guideline 303 A. Two model activated sludge units with industrial sludge were run in parallel. After an adaptation phase of 2 weeks (2 and 5 mg DOC/L) the concentration of the test item was increased to 10 mg DOC/L, respectively. The units were run for 5 weeks. The mean degradation was determined based on daily relative elimination of the test item starting after the adaptation phase for 30 consecutive days. The mean daily degradation rate was 75% (ultimate degradation).

 

The degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid was studied in an OECD confirmatory test. Two model activated sludge units with industrial sludge were run in parallel. After an adaptation phase of 2 weeks (1 and 2 mg a.i./L) the concentration of the test item was increased to 10 or 20 mg a.i./L, respectively. The units were run for 8 weeks.

The degradation of the test item was determined using the Orange II method (Few, A. V., Ottewill, R. H.: J. Colloid. Sci, 11 (1956) 34; Scott, G. V.: Anal. Chem., 40 (1968) 768).

The mean degradation was determined based on daily relative elimination of the test item starting after the adaptation phase for 21 consecutive days. The mean daily degradation rate was 98% (primary degradation).