Amines, N-C12–14 (even numbered)-alkyltrimethylenedi-, reaction products with chloroacetic acid and diethylenetriamine, N-C12–14 (even numbered)-alkyl derivs.

Biodegradation in water and sediment: simulation tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: sewage treatment simulation testing

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)

GLP compliance:

no

Radiolabelling:

no

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial, adapted

Duration of test (contact time):

8 wk

Initial conc.:

5 mg/L

Based on:

act. ingr.

Initial conc.:

10 mg/L

Based on:

act. ingr.

Parameter followed for biodegradation estimation:

test mat. analysis

Details on study design:

- 2 weeks adaptation phase: 2 units with 1 and 2 mg a.i./L
- afterwards the laboratory units were run for 8 weeks

% Degr.:

98

St. dev.:

0.8

Parameter:

test mat. analysis

Sampling time:

18 d

Remarks on result:

other: mean daily degradation rate measured from day 18 to day 39

Transformation products:

not measured

	5 mg a.i./L			10 mg a.i./L
Adaptation phase	mg a.i./L ininflow	mg a.i./L ineffluent	% degradation	mg a.i./L ininflow	mg a.i./L ineffluent	% degradation
1	5.2	1.4	73	11.0	3.9	65
2	6.1	0.9	85	9.2	2.9	68
3	6.3	2.3	63	8.9	2.8	69
5	5.0	1.0	80	10.1	2.5	75
8	5.1	1.2	76	10.0	2.2	78
12	6.0	0.7	88	11.0	0.8	93
13	6.8	0.9	87	10.1	1.7	83
14	5.2	0.6	88	9.8	1.6	84
15	4.9	0.5	90	10.0	1.6	84
16	5.7	0.5	91	11.0	1.4	87
17	5.3	0.5	91	12.0	1.0	92
Evaluation phase
18	5.2	0.2	96	9.8	0.5	95
19	5.4	0.2	96	9.8	0.3	97
20	4.9	0.2	96	10.1	0.3	97
22	5.5	0.1	98	10.2	0.3	97
24	6.0	0.2	97	10.3	0.1	99
26	5.8	0.2	97	11.0	0.1	99
28	5.0	0.1	98	9.5	0.1	99
29	4.9	0.1	98	9.8	0.2	98
31	5.1	0.1	98	10.0	0.1	99
33	5.3	0.1	98	10.1	0	100
34	5.2	0.1	98	9.5	0.1	99
35	5.0	0	100	11.2	0.1	99
36	6.1	0	100	10.2	0	100
37	4.9	0	100	10.0	0.1	99
39	5.2	0	100	9.2	0	100

Validity criteria fulfilled:

not specified

Conclusions:

In an OECD confirmatory test, the degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid (5 and 10 mg a.i./L using industrial adapted sludge) was determined to be 98%.

Executive summary:

The degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid was studied in an OECD confirmatory test.

Two model activated sludge units with industrial sludge were run in parallel. After an adaptation phase of 2 weeks (1 and 2 mg a.i./L) the concentration of the test item was increased to 10 or 20 mg a.i./L, respectively. The units were run for 8 weeks.

The degradation of the test item was determined using the Orange II method (Few, A. V., Ottewill, R. H.: J. Colloid. Sci, 11 (1956) 34; Scott, G. V.: Anal. Chem., 40 (1968) 768).

The mean degradation was determined based on daily relative elimination of the test item starting after the adaptation phase for 21 consecutive days. The mean daily degradation rate was 98%.

Reference 2

Endpoint:

biodegradation in water: sewage treatment simulation testing

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)

GLP compliance:

no

Radiolabelling:

no

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial, adapted

Duration of test (contact time):

8 wk

Initial conc.:

10 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

- 2 weeks adaptation phase: 2 units with 2 and 5 mg DOC/L
- afterwards the laboratory units were run for 5 weeks

% Degr.:

75

St. dev.:

4.8

Parameter:

test mat. analysis

Sampling time:

18 d

Remarks on result:

other: mean daily degradation rate measured from day 18 to day 48

Transformation products:

not measured

Adaptation phase	mg DOC/L in inflow	mg DOC/L in effluent	% degradation
1	15	8.2	22.3
2	16.8	10.6	29.1
3	13.5	7.9	36
5	12.3	8.6	57.7
8	13.3	10.1	63.4
12	13.5	10.8	69.1
13	12.3	9.6	69.1
15	10.6	7.7	66.9
17	11.1	7.7	61.1
Evaluation phase
18	13.3	10.1	63.4
19	12.3	8.6	57.7
21	13.5	10.8	69.1
23	12	9.7	73.7
24	12.3	9.6	69.1
26	9.1	7.9	86.3
27	10.6	7.7	66.9
29	10.1	8.2	78.3
31	11.1	8.6	71.4
32	9.6	7.7	78.3
34	9.1	8.4	92
35	8.9	7.9	88.6
37	11.1	7.7	61.1
39	9.6	6.8	68
40	14	10.8	63.4
42	11.5	8.6	66.9
43	12	10.6	84
44	11.5	10.6	89.7
46	11.1	10.1	88.6
47	9.6	8.2	84
48	10.6	8.6	77.1

Validity criteria fulfilled:

not specified

Conclusions:

The degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid (10 mg DOC/L using industrial adapted sludge) was determined to be 75%.

Executive summary:

The degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid was studied in a coupled units etst according to OECD Guideline 303 A..

Two model activated sludge units with industrial sludge were run in parallel. After an adaptation phase of 2 weeks (2 and 5 mg DOC/L) the concentration of the test item was increased to 10 mg DOC/L, respectively. The units were run for 5 weeks.

The mean degradation was determined based on daily relative elimination of the test item starting after the adaptation phase for 30 consecutive days. The mean daily degradation rate was 75%.

Description of key information

degradation rate 75% (ultimate degradation) after adaptation

Additional information

The degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid was studied in a coupled units test according to OECD Guideline 303 A. Two model activated sludge units with industrial sludge were run in parallel. After an adaptation phase of 2 weeks (2 and 5 mg DOC/L) the concentration of the test item was increased to 10 mg DOC/L, respectively. The units were run for 5 weeks. The mean degradation was determined based on daily relative elimination of the test item starting after the adaptation phase for 30 consecutive days. The mean daily degradation rate was 75% (ultimate degradation).

 

The degradation of Reaction product of lauryl-PDA/lauryl-DETA with chloroacetic acid was studied in an OECD confirmatory test. Two model activated sludge units with industrial sludge were run in parallel. After an adaptation phase of 2 weeks (1 and 2 mg a.i./L) the concentration of the test item was increased to 10 or 20 mg a.i./L, respectively. The units were run for 8 weeks.

The degradation of the test item was determined using the Orange II method (Few, A. V., Ottewill, R. H.: J. Colloid. Sci, 11 (1956) 34; Scott, G. V.: Anal. Chem., 40 (1968) 768).

The mean degradation was determined based on daily relative elimination of the test item starting after the adaptation phase for 21 consecutive days. The mean daily degradation rate was 98% (primary degradation).