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EC number: 218-451-9 | CAS number: 2155-60-4
The test substance was checked for possible direct MTT reduction by adding the test substance to MTT medium. Because no colour change was observed it was concluded that the test substance did not interact with MTT. The standard deviation value of the percentage viability of three tissues treated identically was less than 9%, indicating that the test system functioned properly.
In an in vitro skin irritation test using a human skin model (EPISKIN Small Model), the influence of the test substance on the viability of human skin was tested. The test substance was applied directly to 0.38 cm2 cultured skin (25 μl). After 15 minutes, the substance was removed and cells were cultured for 42 hours. The viability of the cells was tested by reduction of MTT. Survival of unexposed skin was set at 100%, the positive control had a mean cell viability of 7% whereas the test substance showed cell viability of 120%. Since the mean relative tissue viability after exposure to the test substance was above 50%, it can be concluded that dibutyl itaconate is not irritating in the in vitro skin irritation test.
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