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EC number: 214-675-6 | CAS number: 1184-78-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, however, no GLP, no analytical monitoring
Cross-reference
- Reason / purpose for cross-reference:
- read-across source
Reference
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- See attached document in section 13 "Assessment reports" for justification and rationale of the analogy approach
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 190.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 115.06 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the analogy approach, the target substance and source substance are expected to have same behavior in aquatic compartment. Hence, the EC50 values were derived for the trimethylamine N-oxyde from experimental results on the souce substance trimethylamine. The EC50 for the target substance were defined at 190.5 and 115.6 mg/L, respectively based on growth rate and biomass.
- Executive summary:
Based on their physico-chemical properties, the two substances showed similarity on their structure except the oxygen in the TMAO. Both of the two substances are soluble in water. The partition coefficient n-octanol and water values for the two substances are lower than 0. The two substances could be found in environmental aqueous compartments due to their physico-chemical properties. Additionally, Henry’s law constant were calculated. The transfer from water to air compartments is considered to be slow, even negligible. QSAR were performed on TMAO (ready biodegradability and toxicity to fish, daphnia and algae). Similar results were found for target compared to experimental values obtained on the source substance : readily biodegradable (by Biowin and Vega), fish acute toxicity > 100 mg/L (Ecosar and Vega), daphnia acute toxicity: 10-100 mg/L (Ecosar and Vega), algae acute toxicity >100 mg/L (Ecosar), daphnia chronic.
The algae toxicity is expected to be the same for the two substances.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412, part 9
- GLP compliance:
- no
Test material
- Reference substance name:
- Trimethylamine
- EC Number:
- 200-875-0
- EC Name:
- Trimethylamine
- Cas Number:
- 75-50-3
- Molecular formula:
- C3H9N
- IUPAC Name:
- N,N-dimethylmethanamine
- Details on test material:
- trimethylamine in 45 % aqueous solution
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: 86.81
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- no data
Test conditions
- Hardness:
- no data
- Test temperature:
- 20 °C
- pH:
- uninoculated: 8.1 - 8.5
inoculated: 8.5 - 9.9 - Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- Nominal test concentrations: 7.81, 15.83, 31.25, 82.5, 125, 250, 500 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks (250 ml) plugged with gas permeable silicon-sponge caps
- Type (delete if not applicable): open
- Material: glass
- Fill volume: 100 ml
- Initial cells density: 10 000 cells/ml
- No. of vessels per concentration (replicates): 6
- No. of vessels uninoculated control (replicates): 6
- No. of vessels control (replicates): 1
- medium: OECD-medium
- flasks were incubated in an incubation chamber for 72 h
- flasks were shaken once a day to hold cells in suspension
- illumination: permanent artificial light
- light intensity: approx. 120 µE/(m2*s)
Preparation of stock solution:
1 g test substance/litre (sterile bidest. water; test substance is volatile; fluid appeared clear; conductivity: 13 µS/cm, pH 10.2); stock solution was diluted in test medium to reach the final test concentrations.
- tubes were shaken once a day to hold cells in suspension
- illumination: permanent artificial light
- light intensity: approx. 120 µE/(m2s)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter (in vivo chlorophyll-a fluorescence at 685 nm as a size for the biomass (pulsed excitation with light flashesurements) after 0, 24, 48, 72 and 96 h - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- recalculated
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 86 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- recalculated
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 90.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- recalculated
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 42.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- recalculated
Any other information on results incl. tables
During the 96 h exposure to trimethylamine fluorescence values (given as % of control) were as follows (average of 6 replicates):
Nominal concentration (mg/l) | 24 h | 48 h | 72 h | 96 h |
7.81 | 109.92 | 111.63 | 130.69 | 108.36 |
15.83 | 102.52 |
89.67 | 108.69 |
98.84 |
31.25 | 93.01 | 96.3 | 112.76 | 87.79 |
82.5 | 72.93 | 97.84 | 74.17 | 54 .96 |
125 | 42.28 | 37.17 | 32.54 | 28.37 |
250 | 11.63 | 1.82 | 0.55 | 0.7 |
500 | 0 | -0.13 | 0 | -0.02 |
pH values:
Nominal test concentrations | test start; not inoculated | 96 h; not inoculated | 96 h; inoculated |
control | 8.0 | 8.2 | 9.8 |
7.81 | 9.7 | 8.5 | 8.5 |
15.63 | 9.4 | 8.4 | 8.5 |
31.25 | 9.1 | 8.3 | 9.0 |
62.5 | 8.8 | 8.2 | 9.5 |
125 | 8.5 | 8.2 | 9.9 |
250 | 8.3 | 8.1 | 9.9 |
500 | 8.1 | 8.2 | 9.9 |
Treatment [mg/L] |
Growth rate inhibition [%] |
Biomass decrease [%] |
Yield Decrease [%] |
Control |
0.0 |
0.0 |
0 |
7.81 |
-5.9 |
-23.3 |
-31.1 |
15.63 |
-1.4 |
-1.5 |
-8.8 |
31.25 |
-4.0 |
-5.8 |
-13.0 |
62.50 |
6.5 |
29.2 |
26.1 |
125 |
26.0 |
67.8 |
68.4 |
250 |
122.8 |
102.3 |
100.9 |
500 |
235.8 |
103.1 |
101.1 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
Scenedesmus subspicatus was exposed in this study to trimethylamine. Two effects were investigated based on the growth rate as well as on biomass; values were recalculated; therefore nominal results are reported. The concentration is based on the test material. The test duration was 72 hours. For growth rate an EC50 of 150 mg/L and an EC10 of 86 mg/L is reported. Taken the biomass into account, an EC50 of 90.6 mg/L and an EC10 of 42.6 mg/L is recalculated.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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