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EC number: 214-675-6 | CAS number: 1184-78-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- exposure period: 06. - 10. Dezember 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- GLP guideline study (detailed information in Japanese; basic information in English)
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Trimethylamine
- EC Number:
- 200-875-0
- EC Name:
- Trimethylamine
- Cas Number:
- 75-50-3
- Molecular formula:
- C3H9N
- IUPAC Name:
- N,N-dimethylmethanamine
- Details on test material:
- The test solutions were prepared with trimethylamine hydrochloride (CAS 593-81-7). The test results are given as trimethylamine. The use of the hydrochloride is not expected to have influenced the test results.
Since the test substance (trimethylamine) is a gas at room temperature and difficult to handle, trimethylamine hydrochloride was used as a test substance in consideration of the operability in the test and trimethylamine concentration.
- Purity: 98.4%; The purity was set to 100%.
- Impurities: 2.6% water
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: in darkness / desiccator
- Stability under test conditions: stable under storage conditions
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and test concentration (one test vessel per concentration) at test start (ca. 0 h), before and after water renewal (48h) and at test end (96 h)
- Sampling method: The samples were taken from the middle layer of the test solutions.
- Sample size: approx. 10 ml
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
The required amount of test sample was weighed [converted into trimethylamine = 59.11 / 95.57 (61.85%)] and dissolved in test water to prepare the test stock solution (100 mg/L). The necessary amount of test stock solution and test water were mixed in preparation container and stirred. Test solutions were prepared and divided into test vessels.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no
Test organisms
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Medaka
- Source: General Foundation Corporation Chemical Substance Evaluation Research Organization Kurume office, Fukuoka Prefecture Kurume, Japan
- Age at study initiation (mean and range, SD): 3 months
- Length at study initiation (length definition, mean, range and SD): 2.3 +/-1.2 cm (randomly grouped)
ACCLIMATION
- Acclimation period: 25 days (11. Nov. - 06. Dez. 2010)
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: Bait for carp
- Health during acclimation (any mortality observed): no (0% mortality)
FEEDING DURING TEST
no (no feeding 24 h before test start)
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 44 mg/L (as CaCO3; at test start)
- Test temperature:
- 23.4 - 24.0 °C
- pH:
- 7.7 - 7.9
- Dissolved oxygen:
- 8.2 - 8.4 mg/L
- Salinity:
- not specified
- Conductivity:
- 17 mS/m (at test start)
- Nominal and measured concentrations:
- 0 (control), 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 7.8 L aquarium
- Type (delete if not applicable): closed (transparent plastic lid)
- Material, size, headspace, fill volume: glass, (24 x 28 cm), fill volume: 5 L
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): water renewal after 48 h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Total organic carbon: 0.4 mg/L
- Alkalinity: 35 mg/L
- Intervals of water quality measurement: at test beginning
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 (L:D)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- motality and abnormal symptoms (after 3, 24, 48, 72 and 96 h)
TEST CONCENTRATIONS
- Range finding study : yes
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- copper sulfate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- analytically verified
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- analytically verified
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- analytically verified
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- analytically verified
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: none
- Other adverse effects control: none
- Abnormal responses: none
- Mortality of control:0%
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- 96-h LC50: 0.45 mg/L
Other:
- Exposure period: 15. - 19. Nov. 2010
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Cumulative mortality [%]:
Nominal concentration
[mg/L]
Mortality after 3 h
Mortality after 24 h
Mortality after 48 h
Mortality after 72 h
Mortality after 96 h
0 (control)
0
0
0
0
0
100
0
0
0
0
0
Observed abnormal responses:
No abnormal responses could be observed.
Dissolved oxygen concentration [mg/L]:
Nominal concentration
[mg/L]
at
test start
24 h
48 h
(old)
48 h
(new)
72 h
at
test end
0 (control)
8.4
8.4
8.2
8.4
8.3
8.4
100
8.4
8.3
8.2
8.3
8.2
8.2
old: before water renewal; new: after water renewal
pH:
Nominal concentration
[mg/L]
at
test start
24 h
48 h
(old)
48 h
(new)
72 h
at
test end
0 (control)
7.7
7.7
7.9
7.7
7.8
7.8
100
7.7
7.8
7.8
7.7
7.9
7.7
old: before water renewal; new: after water renewal
Temperature [°C]:
Nominal concentration
[mg/L]
at
test start
24 h
48 h
(old)
48 h
(new)
72 h
at
test end
0 (control)
23.6
23.4
23.9
23.4
23.9
24.0
100
23.6
23.4
23.9
23.6
24.0
24.0
old: before water renewal; new: after water renewal
Measured concentrations of test item in test solution:
Concentration
Measured concentration [mg/L]
(Percentage of measured concentration versus nominal concentration %)
Nominal [mg/L]
at
test start
48 h
(old)
48 h
(new)
at
test end
Geometric mean value
0 (control)
n.d.
n.d.
n.d.
n.d.
---
100
98
(98)
97
(97)
97
(97)
92
(92)
96
(96)
old: before water renewal; new: after water renewal
n.d.: < 1.0 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- An LC50 of > 100 mg/L (24, 48, 72 or 96 h) was determined for Trimethylamine by the Ministry of the Environment in Japan.
- Executive summary:
An LC50 of > 100 mg/L (24, 48, 72 or 96 h) was determined for Trimethylamine by the Ministry of the Environment in Japan.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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