Registration Dossier
Registration Dossier
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EC number: 254-991-1 | CAS number: 40594-65-8
Water solubility
Administrative data
Link to relevant study record(s)
Description of key information
< 1 mg/l, at 20 °C and pH 5.8 -5.9
Key value for chemical safety assessment
Additional information
The water solubility of the test item was determined by using the column elution method according to the OECD Guideline No. 105, EC method A.6 and CIPAC MT 157. The column, loaded with the test item, was eluted with 100 ml of distilled water, and then two tests were performed at 25 ml/h and 12.5 ml/h collecting the eluted fractions every 60 minutes. The pH of water and the pH of each fraction collected were measured to ensure the test item stability in water. Each fraction was analysed using the HPLC analysis. During Test No. 3, the fractions collected at 25.0 ml/h were checked for the presence of undissolved dispersed colloidal matter by examination of the Tyndall effect. As negative control reference, demineralized purified water was checked using a green laser at wavelength of 532 nm ± 10 nm and no Tyndall effect was observed. From the Column Elution Tests No. 1 and 2, performed at 25.0 ml/h and 12.5 ml/h, a mean value of 3.7 μg/ml was obtained, but this value was demonstrated to be overestimated considering both the Tyndall effect observed for a 1:4 dilution in purified water of the eluted fraction (Test No. 3) and the HPLC analysis conducted on three eluted fractions diluted 1:2 in methanol (Test No. 4).
The water solubility of the test item at 20 °C and pH 5.8 -5.9 is lower than 1 mg/l.
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