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EC number: 947-842-3 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10.2004 - 11-2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Initial conc.:
- 50 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Reference substance:
- acetic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 100
- Sampling time:
- 21 d
- Results with reference substance:
- 82 % after 14 days and 100 % after 28 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item reached the 10 % level (beginning of biodegradation) after an adaptation phase of 4 days. The biodegradation was rapid and reached the pass level of 60 % already on day 10. The biodegradation came to a maximum of 100 % after 21 days.
- Executive summary:
The ready biodegradability was determined with a non adapted activated sludge for the test item over a period of 28 days in the Modified Sturm Test. The test item was tested with a concentration of 50 mg/L in duplicates, corresponding to a carbon content (TOC) of 12.4 mgC/L in the test vessels. The biodegradation of the test item was followed by titrimetric analyses of the quantity of CO2 produced by the respiration of bacteria. The degradation was finished on day 28 by acidification; the last titration was made on day 29. The percentage Co2 production was calculated in relation to the theoretical CO2 (ThCo2) of the test item. The biodegradation was calculated for each titration time.
To check the activity of the test system sodium acetate was used as functional control. The percentage degradation of the functional control reached the pass level of 60 % after 8 days. In the toxicity control containing both test and reference item a biodegradation rate of 82 % occurred within 14 days and came to a maximum of 100 % after 28 days. The test item in the toxicity control did not inhibit the biodegradation of the reference item.
The test item reached the 10 % level (beginning of biodegradation) after an adaptation phase of 4 days. The biodegradation was rapid and reached the pass level of 60 % already on day 10. The biodegradation came to a maximum of 100 % after 21 days.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 11.93 - 12.93
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Deviations:
- not specified
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- STP Niederrad
- Duration of test (contact time):
- 28 d
- Initial conc.:
- >= 5 - <= 40 mg/L
- Based on:
- DOC
- Parameter:
- % degradation (DOC removal)
- Value:
- 88
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item is readily biodegradable in 10 -d window 86 % and after 28 d (88%)
- Executive summary:
The test item is readily biodegradable in 10 -d window 86 % and after 28 d (88%)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP guideline study with acceptable restrictions (test performance is briefly reported)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - - Source of inoculum/activated sludge: not specified
- Duration of test (contact time):
- 28 d
- Initial conc.:
- >= 5 - <= 40 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: anorganic nutrient solution containing micronutrients and essential vitamines
- Test temperature: 20 - 25 °C
- Continuous darkness: yes
TEST SYSTEM
- Other: The test solution was placed on a mechanical shaker
SAMPLING
- Sampling frequency: Samples were taken after 7, 14, 21, 27 and 28 days.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes, a trial without the test or reference substance was conducted - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (DOC removal)
- Value:
- 94.4
- Sampling time:
- 28 d
- Details on results:
- The test substance reached a biodegradation of 94.4% within a test period of 28 days. The 10 day-window was met. Thus, the test substance is readily biodegradable according to OECD criteria.
- Results with reference substance:
- The reference substance was biodegraded to 97.9% within 28 days.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2008-10-15 to 2008-11-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study according OECD Guideline and GLP, no deviations from Guideline, biodegradation equal/better than reference item sodium benzoate, toxicity control demonstrated no inhibitory effect of the test item
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 108 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- at 20 mg/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 83 - 86
- Sampling time:
- 28 d
- Details on results:
- Lag time was 1.5 d, Degradation time was 13.5 d, 10d window achieved (10% at day 1.5 and 60% at day 8)
- Results with reference substance:
- validity criteria was met based on degradation curve
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Executive summary:
The biodegradability of the test item Hostapon SG (24,6% a.i. SCG) was investigated in an OECD 301B ready test with non-adapted inoculum over a period of 28d. The initial test item concentration was 108 mg/L. 83 -86% Biodegradation was achieved after 28d. The 10% level of degradation was reached after 1.5d and the 60% pass level after 8d. In a toxicity test with the reference substance sodium benzoate it was demonstrated that the test item in not inhibitory.
Based on the results given before the test item Hostapon SG is readily biodegradable fulfilling the 10d window.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Justification is provided in the separate statement.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 86
- Sampling time:
- 28 d
- Interpretation of results:
- readily biodegradable
- Executive summary:
In three studies with two different supporting substances (structural analogue or surrogate) ready biodegradability could be shown in the 10 -d windows and after the 28 day period.
Referenceopen allclose all
Table 1: Biodegradation of reference and test substance in % DOC removal throughout the test period of 28 days
Days |
Reference substance |
Test substance |
7 |
93.0 |
81.4 |
14 |
97.8 |
90.5 |
21 |
97.2 |
93.0 |
27 |
97.8 |
95.5 |
28 |
97.9 |
94.4 |
In a toxicity test containing both the test item and the reference item >25% biodegradation was achieved within 14d and 84% after 28d. This means that the test item is not inhibitory.
Description of key information
In a Key study with the test item it could be shown that the test item reached the 10 % level (beginning of biodegradation) after an adaptation phase of 4 days. The biodegradation was rapid and reached the pass level of 60 % already on day 10. The biodegradation came to a maximum of 100 % after 21 days.
In three studies with two different supporting substances (structural analogue or surrogate) ready biodegradability could be shown in the 10 -d windows and after the 28 day period.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
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