Fatty acids, coco, esters with 3,3'-oxybis[1,2-propanediol]

Endpoint summary

Description of key information

Key values acute aquatic toxicity

Algae: 72-EC50 (Pseudokirchneriella subcapitata, growth rate) = 17.57 mg test item/L; 72h-NOErC (Pseudokirchneriella subcapitata, growth rate) = 4.42 mg test item/L; 72h-LOErC (Pseudokirchneriella subcapitata, growth rate) = 9.56 mg test item/L (mean measured)

Daphnia: 46h-EC50 (Daphnia magna, immobility) > 26 mg/L (above water solubility); 48h-NOEC (Daphnia magna, immobility) = 24.1 mg test item/L; 48h-LOEC (Daphnia magna, immobility) = 26 mg test item/L (mean measured)

Fish: 96h-LC50 (Danio rerio) > 18.53 mg/L; 96h-NOEC > 18.53 mg/L (geometric mean measured)

Key value STP microorganisms

STP microorganisms: 3h-EC50 (activated sludge, total respiration inhibition) = 597.1 mg/L; 3h-EC10 (activated sludge, total respiration inhibition) = 146.06 mg/L; NOEC (activated sludge, total respiration inhibition) = 100 mg/L (nominal)

Additional information

Acute Aquatic Toxicity

Toxicity studies for the submission substance were available for three trophic levels, i.e. algae, daphnia and fish. The most sensitive organisms were green algae. All studies were considered reliable and adequate for the environmental hazard assessment for aquatic organisms.

Algae

In a reliable key study, toxicity of the submission substance ongrowth of the freshwater green algal was investigated (RL1). Growing cultures of unicellular green algal were exposed to five test concentrations under static test conditions during 72 hours. The corresponding mean measured concentrations were 39.5, 9.56, 4.42 mg test item/L, and the lowest two concentrations of 3.2 and 1.0 mg test item/L were below LOQ. Adverse effects on growth rate and yield were observed at the two highest test concentrations, i.e. 39.5 and 9.56 mg/L test item. Derived effect concentration on yield (y) and growth rate (r) were: 72h-EyC50 = 7.19 mg/L; 72h-LOEyC = 9.56 mg/L; 72h-NOEyC = 4.42 mg/L; 72-ErC50= 17.57 mg/L; 72h-LOErC = 9.56 mg/L; 72h-NOErC = 4.42 mg/L.

Invertebrates

In a reliable key study, acute toxicity of the submission substance on the mobility of Daphnia magna was investigated (RL1). Young daphnids were exposed to five test concentrations (nominal 100, 45.5, 20.7, 9.4 and 4.3 mg/L) for 48 hours in a static test. The test concentrations were analytically verified by analysing the Total Organic Carbon content (TOC). 

After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the test item concentration of 14.6 mg test item/L. One animal was immobile at test concentration of 24.1 mg test item/L (5% effect), and 20 animals were immobile at 44.2 mg test item/L test concentration. Reported effect concentrations refer to mean measured concentrations: 46h-EC50 (Daphnia magna, immobility) > 26 mg/L (above water solubility); 48h-NOEC (Daphnia magna, immobility) = 24.1 mg test item/L; 48h-LOEC (Daphnia magna, immobility) = 26 mg test item/L.

Fish

In a reliable key study, acute toxicity of the submission substance to fish was evaluated according to OECD 203 and in compliance with GLP (RL1). For this purpose, juvenile Zebrafish were exposed to a threshold test concentration for 96 hours in a static test (limit test). A threshold approach was performed in order to demonstrate that the submission substance has no toxic effects on fish up to a threshold concentration, i.e. the lowest derived EC50 value from existing relevant algae or acute invertebrate (e.g. daphnia) tests. No mortality or sublethal effects were observed in neither treatment group nor control. The 96-hour LC50 was determined to be > 18.53 mg test item/L based on geometric mean measured concentrations.

STP microorganisms

In a reliable key study, toxicity of the submission substance on the activity of activated sludge was tested according to OECD 209 and in compliance with GLP (RL1). The respiration rate (oxygen consumption) of an aerobic activated sludge was measured in the presence of five test concentrations (nominal 10, 32, 100, 320 and 1000 mg test item/L) after an incubation period of 3 hours. Total respiration rate of the activated sludge was not inhibited at test concentrations up to 100 mg/L (inhibition below 10%) and moderately inhibited for the two highest test concentrations: For the test item concentrations of 320 mg/L and 1000 mg/L, the inhibition rates were 34.4%, and 64.2%, respectively. Derived nominal effect concentrations were: 3h-EC50 (activated sludge, total respiration inhibition) = 597.1 mg/L; 3h-EC10 (activated sludge, total respiration inhibition) = 146.06 mg/L; NOEC (activated sludge, total respiration inhibition) = 100 mg/L. In conclusion, the test was considered reliable and adequate for the environmental hazard assessment for STP mircoorganisms.