Butanoic acid, 4-amino-4-oxosulfo-, N-coco alkyl derivs., monosodium salts, compds. with triethanolamine

Short-term toxicity to aquatic invertebrates

Description of key information

In an OECD 202 with Daphnia magna the test item reveal after 48 h exposure a toxicity (related to loading rates ) of EL 50 = 26.08 mg/l and an EL10 of 8.08 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

26.6 mg/L

Additional information

 Immobilisation of Daphnia magna exposed to the test item, Efka FA 4600 (solvent free), was investigated during a 48-hour semi-static test. The definitive test was performed with the following filtrates of the test item loading rates of 200, 100, 50, 25, 12.5 and 6.25 mg/L plus the control. The test was performed in glass beakers of 150 mL capacity. Each replicate contained 100 mL of either the filtrate of the test item loading rate or the control. Four replicates were used for each treatment, each with fiveDaphnia magna.

 

The test item is an UVCB substance soluble in water. Since precipitations of the test item were observed in the first preliminary test, the water accommodation fractions (WAFs) approach was used.

The test item loading rates were prepared by mixing the proper amounts of the test item withthe ISO test water.The test item loading rates and the control were filtered through a conditioned (pre-filtering a small volume of either the test loading rate or control to saturate the filter) nitrocellulose membrane filter(0.22 µm,Millipore).After 24 h of exposure, the filtrates of the test item loading rates and the filtrate of the control were renewed.

No immobilisation ofDaphnia magnawas observed during the period of exposure in the filtrate of the control. Immobilisation in the filtrates of the test item loading rates of 6.25, 12.5, 25, 50, 100 and 200 mg/L were 5, 20, 55, 70, 90 and 100%, respectively. Inthe filtrate of the control and in filtrate of the test item loading rate of 6.25 mg/L no abnormal behaviour ofDaphnia magnawas observed. In the filtrates of the higher test item loading rates slow movement ofDaphnia magnawas observed. In each filtrate of the test item loading ratesDaphnia magnawas covered with particles of the test item. In the filtrate of the test item loading rate of 200 mg/L precipitation of the test item was observed.

Since the test item is an UVCB, treatments analyses were performed usingtotal organic carbon (TOC) assum parameter.The total organic carbon analyses were performed in samples of each filtrate of a loading rate and the control at exposure initiation, at renewal and at exposure termination.

 

In the control, the analysed total organic carbon concentration remained below the LoQ value (<1.0 mg C/L)at exposure initiation, at renewal (fresh and old samples) and at exposure termination.

In the filtrate of the test item loading rates of 6.25, 12.5, 25, 50, 100, 200 mg/L, the analysed total organic carbon concentration was 1.5, 3.6, 6.3, 12.4, 21.0, 42.0 mg C/L, at exposure initiation, 1.5, 3.9, 6.7, 11.0, 19.3, 40.7 mg C/L at renewal in fresh samples, 1.6, 3.7, 6.4, 11.8, 20.2, 42.1 mg C/L in old samples and 1.5, 3.6, 6.7, 9.6, 18.2, 38.3 mg C/L at exposure termination, respectively.

 

 Results:

The endpoint valuesdetermined based on the nominal test item loading ratesare given below:

ELR50(48h) is 26.68 mg/L (95% confidence interval 20.02 – 35.14 mg/L).