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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
Not reported
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Read across study
Justification for type of information:
Refer to section 13 of IUCLID for details on the read-across justification.
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Maximal soluble amount of test material (saturation concentration) diluted in doubly distilled water. Test concentrations prepared by serial dilution of initial saturated solution.

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida MIGULA, strain Berlin 33/2, from the German Microorganism culture Collection (D-3300 Braunschweig)

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Test temperature:
25 ±1 °C
Nominal and measured concentrations:
Nominal: 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L

Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
6 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
830 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the study, the EC50 and EC10 were 6.0 and 0.83 g/L (i.e. 6000 and 830 mg/L), respectively.
Executive summary:

A study was conducted to assess the toxicity of the read across substance, Amides, C8-18 (even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl), to the bacteria Pseudomonas putida according to DIN Guideline 38412 L8, in compliance with GLP. Pseudomonas putida colonies, in triplicate, were exposed to the test substance at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 72 h. Under the conditions of the study, the EC50 and EC10 were 6.0 and 0.83 g/L (i.e. 6000 and 830 mg/L), respectively (Poth, 1992).

Description of key information

Key value for chemical safety assessment

EC50 for microorganisms:
6 000 mg/L
EC10 or NOEC for microorganisms:
830 mg/L

Additional information

A study was conducted to assess the toxicity of the read across substance, amides, C8-18 (even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl), to the bacteria Pseudomonas putida according to DIN Guideline 38412 L8, in compliance with GLP. Pseudomonas putida colonies, in triplicate, were exposed to the test substance at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 72 h. Under the conditions of the study, the EC50 and EC10 were 6.0 and 0.83 g/L (i.e. 6000 and 830 mg/L), respectively (Poth, 1992).