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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
The substance was tested carring out a OECD 301B study, together with the other representative samples of sulfated oils, which this read-across record refers to. Unfortunately, as stated by the Laboratory "For 7 of the 8 substances we could determine the carbon content, so that we are able to conduct the studies acc. to OECD 301 B. Only for CNH [Oils, vegetable, sulfated, ammonium salts - Editor's Note], we could not determine the carbon content because flakes were formed during analysis" (ref.: email, 2012-02-12, from RTC Lab). For this reason, an exeperimental study is not available, but given the structural similarities of the substance against the other representative samples of sulphated oils, it was not considered necessary to run again the study. Fo rmore details on the structural similarities of the sulphated oils, refer to section 13.2
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
no
Specific details on test material used for the study:
Seven test materials, analogues of the substance were analysed:
Oils, fish, sulfated, sodium salts (P2)
Rape oil, sulfated, sodium salt (CP12)
Rape oil, sulfated, sodium salt (C5)
Rape oil, sulfated, sodium salt (CSO3)
Rape oil, sulfated, sodium salt (CSO5)
Castor oil, sulfated, sodium salt (R3)
Rape oil, sulfated, compd. with ethanolammine (CTEA)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L
Initial conc.:
20 mg/L
Based on:
test mat.
Remarks:
P2
Initial conc.:
15 mg/L
Based on:
test mat.
Remarks:
CP12
Initial conc.:
20 mg/L
Based on:
test mat.
Remarks:
C5
Initial conc.:
15 mg/L
Based on:
test mat.
Remarks:
CSO3
Initial conc.:
15 mg/L
Based on:
test mat.
Remarks:
CSO5
Initial conc.:
20 mg/L
Based on:
test mat.
Remarks:
R3
Initial conc.:
15 mg/L
Based on:
test mat.
Remarks:
CTEA
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.8 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles

TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0 125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation proces ses. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control
Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (CO2 evolution)
Value:
64
Sampling time:
28 d
Remarks on result:
other: test mat. P2
Parameter:
% degradation (CO2 evolution)
Value:
61
Sampling time:
28 d
Remarks on result:
other: test mat. CP12
Parameter:
% degradation (CO2 evolution)
Value:
85
Sampling time:
28 d
Remarks on result:
other: test mat. C5
Parameter:
% degradation (CO2 evolution)
Value:
66
Sampling time:
28 d
Remarks on result:
other: test mat. CSO3
Parameter:
% degradation (CO2 evolution)
Value:
63
Sampling time:
28 d
Remarks on result:
other: test mat. CSO5
Parameter:
% degradation (CO2 evolution)
Value:
62
Sampling time:
28 d
Remarks on result:
other: test mat. R3
Parameter:
% degradation (CO2 evolution)
Value:
63
Sampling time:
28 d
Remarks on result:
other: test mat. CTEA
Details on results:
The % degradation for the tested sulfated oils resulted to be >60%. The highest value obtained of 85% is related to the sample with the higher fraction of free fatty acids.
Results with reference substance:
60% after 7 days, 86% after 28 days
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The biodegradability of the representative samples of the sulfated oils has been investigated using the CO2 eviolution test method, performed according to OECD Test Guidelines OECD 301B.
The results obtained demonstrate that the substance is readily biodegradable.
Executive summary:

The biodegradability of the representative samples of the sulfated oils has been investigated using the CO2 eviolution test method, performed according to OECD Test Guidelines OECD 301B.

The results obtained demonstrate that the substance is readily biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L
Duration of test (contact time):
28 d
Initial conc.:
20 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.8 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles

TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control

Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (CO2 evolution)
Value:
64
Sampling time:
28 d
Results with reference substance:
60% after 7 days, 86% after 28 days

Day

% Biodegradation

 

Positive control

Toxicity control

Test substance

Test substance

1

0

1

1

0

4

36

20

27

24

6

57

38

45

41

8

67

55

54

51

11

74

65

59

59

14

79

69

63

63

18

82

71

64

63

22

83

71

64

63

25

83

71

64

63

28

84

71

64

63

29 *

86

71

65

63

* = Remaining 2 gas wash bottles

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
Executive summary:

Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L
Duration of test (contact time):
28 d
Initial conc.:
15 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.7 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles

TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control

Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (CO2 evolution)
Value:
61
Sampling time:
28 d
Results with reference substance:
60% after 7 days, 86% after 28 days

Day

% Biodegradation

 

Positive control

Toxicity control

Test substance

Test substance

1

0

0

0

0

4

36

21

23

27

6

57

42

42

48

8

67

57

50

58

11

74

67

57

64

14

79

71

57

64

18

82

71

57

64

22

83

71

57

64

25

83

71

57

64

28

84

71

57

64

29 *

86

71

57

64

* = Remaining 2 gas wash bottles

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
Executive summary:

Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L
Duration of test (contact time):
28 d
Initial conc.:
20 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.7 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles

TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control

Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (CO2 evolution)
Value:
85
Sampling time:
28 d
Results with reference substance:
60% after 7 days, 86% after 28 days

Day

% Biodegradation

 

Positive control

Toxicity control

Test substance

Test substance

1

0

0

1

1

4

36

21

25

28

6

57

40

42

45

8

67

59

55

59

11

74

72

61

71

14

79

79

65

80

18

82

84

68

91

22

83

89

70

96

25

83

94

70

96

28

84

97

70

97

29 *

86

99

71

98

* = Remaining 2 gas wash bottles

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
Executive summary:

Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L
Duration of test (contact time):
28 d
Initial conc.:
15 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.8 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles

TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control

Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (CO2 evolution)
Value:
66
Sampling time:
28 d
Results with reference substance:
60% after 7 days, 86% after 28 days

Day

% Biodegradation

 

Positive control

Toxicity control

Test substance

Test substance

1

0

0

0

0

4

36

20

29

28

6

57

41

48

48

8

67

59

59

60

11

74

67

66

65

14

79

72

67

65

18

82

73

67

65

22

83

73

67

65

25

83

73

67

65

28

84

73

67

65

29 *

86

73

67

65

* = Remaining 2 gas wash bottles

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
Executive summary:

Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L
Duration of test (contact time):
28 d
Initial conc.:
15 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.7 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles

TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control

Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (CO2 evolution)
Value:
63
Sampling time:
28 d
Results with reference substance:
60% after 7 days, 86% after 28 days

Day

% Biodegradation

 

Positive control

Toxicity control

Test substance

Test substance

1

0

1

0

2

4

36

21

26

27

6

57

43

43

48

8

67

59

53

58

11

74

70

58

61

14

79

74

60

66

18

82

75

60

66

22

83

75

60

66

25

83

75

60

66

28

84

75

60

66

29 *

86

75

61

66

* = Remaining 2 gas wash bottles

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
Executive summary:

Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L
Duration of test (contact time):
28 d
Initial conc.:
20 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.8 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles

TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control

Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (CO2 evolution)
Value:
62
Sampling time:
28 d
Results with reference substance:
60% after 7 days, 86% after 28 days

Day

% Biodegradation

 

Positive control

Toxicity control

Test substance

Test substance

1

0

1

0

0

4

36

21

15

26

6

57

41

35

45

8

67

55

52

54

11

74

62

59

63

14

79

65

59

64

18

82

65

59

64

22

83

65

59

64

25

83

65

59

64

28

84

65

59

64

29 *

86

65

59

64

* = Remaining 2 gas wash bottles

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
Executive summary:

Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L
Duration of test (contact time):
28 d
Initial conc.:
15 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.7 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles

TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control

Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (CO2 evolution)
Value:
63
Sampling time:
28 d
Results with reference substance:
60% after 7 days, 86% after 28 days

Day

% Biodegradation

 

Positive control

Toxicity control

Test substance

Test substance

1

0

1

0

1

4

36

22

25

30

6

57

43

40

48

8

67

61

50

58

11

74

72

56

65

14

79

76

58

68

18

82

76

58

68

22

83

76

58

68

25

83

76

58

68

28

84

76

58

68

29 *

86

77

58

68

* = Remaining 2 gas wash bottles

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
Executive summary:

Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.

Description of key information

Biodegradation - Readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

A number of tests of ready biodegradability have been undertaken with representative samples of sulfated oils. A technical problem during the test of the substance "oils, vegetable, sulfated, ammonium salts" occured and it was not possible to finish the test. However, for all samples tested the results obtained demonstrate that sulfated oils are readily biodegradable.