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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1975
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
abstract
Qualifier:
no guideline available
Version / remarks:
In 1975 no guideline was available
Principles of method if other than guideline:
The test procedures used follows closely those of Lennon and Walker (1964) and Taras (1971)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Remarks:
acetone
Details on test solutions:
The purified materials were weighed on an electrobalance and dissolved in acetone.
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: Federal hatcheries
- Weight at study initiation: 1 to 1.5 g
- Maintained: according to standard procedures of the Fish Control Laboratory (Hunne t al. 1968)

ACCLIMATION
- Acclimation period: yes
- Acclimation conditions: same as test conditions
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
40 - 48 mg/L as CaCO3
Test temperature:
12 ° C
pH:
6.5
Nominal and measured concentrations:
not specified
Details on test conditions:
TEST SYSTEM
- Test vessel: glass jars
- Fill volume: 15 Liters
- Aeration: only before test start
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Soft, reconstituted water

OTHER TEST CONDITIONS
- Adjustment of pH: yes, with 10 mL 1M NaOH and 30 mL 1M KH2PO4
The pH was readjusted with chemical buffers at 24-h intervals as necessary to maintain the selected pH ± 0.2 units

EFFECT PARAMETERS MEASURED:
Mortalities were recorded at 1, 3 and 6 h on the first day of exposure and daily thereafter
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % confidence interval: 0.744 - 1.63 mg/L
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
1.26 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % confidence interval: 1.13 - 1.39 mg/L
Details on results:
not specified
Reported statistics and error estimates:
The methods of Litchfield and Wilcoxon (1949) were used in computation of the LC50's (concentrations producing 50% mortality) and 95% confidence intervals.
Validity criteria fulfilled:
yes
Conclusions:
In a static test with Rainbow trout the LC50 value after 96 hours was determined to be 1.10 mg/L.
Executive summary:

In a static test the effect of the test item on the mortality of Rainbow trout (Salmo gairdneri) was tested. At pH 6.5 an a temperature of 12 °C the LC50 value was determined to be 1.10 mg/L after 96 hours with a 95 % confidence interval of 0.744 - 1.63 mg/L.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1975
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
abstract
Qualifier:
no guideline available
Version / remarks:
In 1975 no guideline was available
Principles of method if other than guideline:
The test procedures used follows closely those of Lennon and Walker (1964) and Taras (1971)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Remarks:
acetone
Details on test solutions:
The purified materials were weighed on an electrobalance and dissolved in acetone.
Test organisms (species):
Ictalurus punctatus
Details on test organisms:
TEST ORGANISM
- Common name: Channel catfish
- Source: Federal hatcheries
- Weight at study initiation: 1 to 1.5 g
- Maintained: according to standard procedures of the Fish Control Laboratory (Hunne t al. 1968)

ACCLIMATION
- Acclimation period: yes
- Acclimation conditions: same as test conditions
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
40 - 48 mg/L as CaCO3
Test temperature:
12 ° C
pH:
6.5
Nominal and measured concentrations:
not specified
Details on test conditions:
TEST SYSTEM
- Test vessel: glass jars
- Fill volume: 15 Liters
- Aeration: only before test start
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Soft, reconstituted water

OTHER TEST CONDITIONS
- Adjustment of pH: yes, with 10 mL 1M NaOH and 30 mL 1M KH2PO4
The pH was readjusted with chemical buffers at 24-h intervals as necessary to maintain the selected pH ± 0.2 units

EFFECT PARAMETERS MEASURED:
Mortalities were recorded at 1, 3 and 6 h on the first day of exposure and daily thereafter
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.632 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % confidence interval: 0.584 - 0.684 mg/L
Details on results:
not specified
Reported statistics and error estimates:
The methods of Litchfield and Wilcoxon (1949) were used in computation of the LC50's (concentrations producing 50% mortality) and 95% confidence intervals.
Validity criteria fulfilled:
yes
Conclusions:
In a static test with Channel catfish the LC50 value after 96 hours was determined to be 0.632 mg/L.
Executive summary:

In a static test the effect of the test item on the mortality of Channel catfish (Ictalurus punctatus) was tested. At pH 6.5 an a temperature of 12 °C the LC50 value was determined to be 0.632 mg/L after 96 hours with a 95 % confidence interval of 0.584 - 0.684 mg/L.

Description of key information

In a static test with Rainbow trout the LC50 value after 96 hours was determined to be 1.10 mg/L.

In a static test with Channel catfish the LC50 value after 96 hours was determined to be 0.632 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.632 mg/L

Additional information

In a static test the effect of the test item on the mortality of Rainbow trout (Salmo gairdneri) was tested. At pH 6.5 and a temperature of 12 °C the LC50 value was determined to be 1.10 mg/L after 96 hours with a 95 % confidence interval of 0.744 - 1.63 mg/L.

In a static test the effect of the test item on the mortality of Channel catfish (Ictalurus punctatus) was tested. At pH 6.5 and a temperature of 12 °C the LC50 value was determined to be 0.632 mg/L after 96 hours with a 95 % confidence interval of 0.584 - 0.684 mg/L.