α,α,α-trifluoro-4-nitro-m-cresol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1975

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

abstract

Qualifier:

no guideline available

Version / remarks:

In 1975 no guideline was available

Principles of method if other than guideline:

The test procedures used follows closely those of Lennon and Walker (1964) and Taras (1971)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Remarks:

acetone

Details on test solutions:

The purified materials were weighed on an electrobalance and dissolved in acetone.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Federal hatcheries
- Weight at study initiation: 1 to 1.5 g
- Maintained: according to standard procedures of the Fish Control Laboratory (Hunne t al. 1968)

ACCLIMATION
- Acclimation period: yes
- Acclimation conditions: same as test conditions

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

40 - 48 mg/L as CaCO3

Test temperature:

12 ° C

pH:

6.5

Nominal and measured concentrations:

not specified

Details on test conditions:

TEST SYSTEM
- Test vessel: glass jars
- Fill volume: 15 Liters
- Aeration: only before test start
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Soft, reconstituted water

OTHER TEST CONDITIONS
- Adjustment of pH: yes, with 10 mL 1M NaOH and 30 mL 1M KH2PO4
The pH was readjusted with chemical buffers at 24-h intervals as necessary to maintain the selected pH ± 0.2 units

EFFECT PARAMETERS MEASURED:
Mortalities were recorded at 1, 3 and 6 h on the first day of exposure and daily thereafter

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % confidence interval: 0.744 - 1.63 mg/L

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

1.26 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % confidence interval: 1.13 - 1.39 mg/L

Details on results:

not specified

Reported statistics and error estimates:

The methods of Litchfield and Wilcoxon (1949) were used in computation of the LC50's (concentrations producing 50% mortality) and 95% confidence intervals.

Validity criteria fulfilled:

yes

Conclusions:

In a static test with Rainbow trout the LC50 value after 96 hours was determined to be 1.10 mg/L.

Executive summary:

In a static test the effect of the test item on the mortality of Rainbow trout (Salmo gairdneri) was tested. At pH 6.5 an a temperature of 12 °C the LC50 value was determined to be 1.10 mg/L after 96 hours with a 95 % confidence interval of 0.744 - 1.63 mg/L.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1975

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

abstract

Qualifier:

no guideline available

Version / remarks:

In 1975 no guideline was available

Principles of method if other than guideline:

The test procedures used follows closely those of Lennon and Walker (1964) and Taras (1971)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Remarks:

acetone

Details on test solutions:

The purified materials were weighed on an electrobalance and dissolved in acetone.

Test organisms (species):

Ictalurus punctatus

Details on test organisms:

TEST ORGANISM
- Common name: Channel catfish
- Source: Federal hatcheries
- Weight at study initiation: 1 to 1.5 g
- Maintained: according to standard procedures of the Fish Control Laboratory (Hunne t al. 1968)

ACCLIMATION
- Acclimation period: yes
- Acclimation conditions: same as test conditions

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

40 - 48 mg/L as CaCO3

Test temperature:

12 ° C

pH:

6.5

Nominal and measured concentrations:

not specified

Details on test conditions:

TEST SYSTEM
- Test vessel: glass jars
- Fill volume: 15 Liters
- Aeration: only before test start
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Soft, reconstituted water

OTHER TEST CONDITIONS
- Adjustment of pH: yes, with 10 mL 1M NaOH and 30 mL 1M KH2PO4
The pH was readjusted with chemical buffers at 24-h intervals as necessary to maintain the selected pH ± 0.2 units

EFFECT PARAMETERS MEASURED:
Mortalities were recorded at 1, 3 and 6 h on the first day of exposure and daily thereafter

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.632 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % confidence interval: 0.584 - 0.684 mg/L

Details on results:

not specified

Reported statistics and error estimates:

The methods of Litchfield and Wilcoxon (1949) were used in computation of the LC50's (concentrations producing 50% mortality) and 95% confidence intervals.

Validity criteria fulfilled:

yes

Conclusions:

In a static test with Channel catfish the LC50 value after 96 hours was determined to be 0.632 mg/L.

Executive summary:

In a static test the effect of the test item on the mortality of Channel catfish (Ictalurus punctatus) was tested. At pH 6.5 an a temperature of 12 °C the LC50 value was determined to be 0.632 mg/L after 96 hours with a 95 % confidence interval of 0.584 - 0.684 mg/L.

Description of key information

In a static test with Rainbow trout the LC50 value after 96 hours was determined to be 1.10 mg/L.

In a static test with Channel catfish the LC50 value after 96 hours was determined to be 0.632 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.632 mg/L

Additional information

In a static test the effect of the test item on the mortality of Rainbow trout (Salmo gairdneri) was tested. At pH 6.5 and a temperature of 12 °C the LC50 value was determined to be 1.10 mg/L after 96 hours with a 95 % confidence interval of 0.744 - 1.63 mg/L.

In a static test the effect of the test item on the mortality of Channel catfish (Ictalurus punctatus) was tested. At pH 6.5 and a temperature of 12 °C the LC50 value was determined to be 0.632 mg/L after 96 hours with a 95 % confidence interval of 0.584 - 0.684 mg/L.