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EC number: 224-658-5 | CAS number: 4439-24-1
In all test bacteria, colony numbers in the test material conditions were less than double the colony numbers of the negative control in both the presence and absence of metabolic activation. No bacterial contamination was detected in the test substance solution or S9 mix. Mutagenicity of the positive control was detected in all of the test bacteria. Furthermore, the value of the positive and negative controls was within the range of background data (average ±3 x standard deviation).
A reverse mutation study was performed to test the effect of 2-iso-butoxyethanol in Salmonella typhimurium TA100, TA1535, TA98, and TA1537 and Escherichia coli WP2 uvrA. The test procedure was according to Good Laboratory Practise (GLP) and the Japanese Methods of Testing New Chemical Substances (2011) and Japanese Standard Concerning Testing Laboratories Implementing Tests for New Chemical Substances (2011) without deviation. This methodology is considered to be similar / equivalent to OECD Guideline 471 (Bacterial Reverse Mutation Assay). The purpose of the experiment was to determine the mutagenic potential of 2-iso-butoxyethanol with and without metabolic activation (S9 mix) using the preincubation technique described by Maron and Ames (1983).
A dose finding test was performed at 1.50, 5.00, 15.0, 50.0, 150, 500, 1500 and 5000 μl/plate of the registered substance. No inhibition of bacterial growth was observed. Two subsequent tests were performed as part of the main experiment using 313, 625, 1250, 2500, and 5000 μl/plate of 2-iso-butoxyethanol. 0.1 ml distilled water (solvent) was used as a negative control and 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide, sodium azide, 9-aminoacridine, benzo[a]pyrene, and 2-aminoanthracene were selected as positive controls. Cultivation was permitted for 48 hours at 37°C. Where the sum of mutated colonies exposed to 2-iso-butoxyethanol was more than twice that of the negative control and where reproducibility or dose-dependency was detected, the registered substance could be categorised as a mutagen.
Reverse mutation inS. typhimuriumandE. colistrains revealed consistently negative results whereby colony numbers at all concentrations were less than double those of the negative control with and without metabolic activation. No bacterial contamination was detected in the solution containing 2-iso-butoxyethanol, nor the S9 mix, and mutagenicity arising from exposure to the positive controls was detected in all bacteria. In addition, the value of the positive and negative controls was within the range of background data (average ±3 x SD). It can be concluded, therefore, that 2-iso-butoxyethanol does not have the potential for genetic toxicity under these experimental conditions.
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