Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
31 January 2018 - 15 February 2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Dilution series
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): Substance observed to be soluble in water.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Dapnia magna
- Source: Eco Cheshire Solutions
- Age of parental stock (mean and range, SD): Not reported
- Feeding during test: Animals were not fed during the test
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 °C +/-2 °C, Constant within 1 °C
pH:
6-9
Dissolved oxygen:
>3 mg/l
Conductivity:
1.15 µS/cm
Nominal and measured concentrations:
Nominal concentrations: 100, 320, 1000, 3200 and 10,000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: borosilicate glass crystallising dishes
- Type: closed
- Material, size, headspace, fill volume: 50 ml of test medium placed in 100 ml capacity test vessel
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO Reconstituted Test Water without additives
- Conductivity: 1.15 µS/cm
- pH: 7.56
- Temperature 18.7C
- Dissolved oxygen 9.15 mg/L
- Intervals of water quality measurement: 0 and 48h

OTHER TEST CONDITIONS
- Adjustment of pH: If, at 0h, the pH of the dilution water was out-with the pH range of 8± 0.3; the pH was returned to within these limits by adjustment with either 1M HCl or NaOH as is appropriate.
- Photoperiod:16h light / 8h dark
- Light intensity: 453 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :No. of immobile animals expressed as a portion of the total exposed. An animal is regarded as immobile if, after gentle stimulation, no movement was visible after 10 seconds. Animals were observed at 24h and 48h.

RANGE-FINDING STUDY
- Test concentrations: 1, 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: 48h EC50 >1000 mg/L
Reference substance (positive control):
yes
Remarks:
potassium dichromate (K2Cr2O7)
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
2 380 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: None reported
- Observations on body length and weight: None reported
- Other biological observations: None reported
- Mortality of control: <10%
- Other adverse effects control: None reported
- Abnormal responses: None reported
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: 24h EC50 0.6-2.1 mg/L
- Dose-response test: 0.32, 0.56, 1.0, 1.8, 3.2 and 100 mg/L
- 24h EC50: 1.27 mg/L
Reported statistics and error estimates:
The result was based on nominal concentrations and was calculated by Linear Interpolation within the CETIS (v1.8) suite of statistical analysis.
Validity criteria fulfilled:
yes
Conclusions:
The 48-hour EC50 was 2100 mg/L and the 48-hour NOEC was 1000 mg/L. Based on the results of this study the substance did not meet the criteria for classification as acutely toxic to the aquatic environment according to Regulation (EC) N° 1272/2008.
Executive summary:

The short-term toxicity of 2-(2-methylpropoxy)ethanol to freshwater crustacean Daphnia magna was determined in a GLP compliant study in accordance with OECD 202 test guideline. A preliminary range-finding test was conducted at concentrations of 1, 10, 100 and 1000 mg/L. The 48h EC50 for the range-finding test was calculated to be >1000 mg/L and as a result the following concentrations were chosen for the definitive test: 100, 320, 1000, 3200 and 10000 mg/L. The 48h EC50 and 48h NOEC were 2100 mg/L and 1000 mg/L respectively. A reference test was conducted concurrently using potassium dichromate. The study met all relevant validity criteria. As the 48-hour EC50 is greater than 1 mg/L, the registered substance is not considered to be a acutely toxic to aquatic invertebrates (CLP Regulation (EC) No 1272/2008).

Description of key information

The short-term toxicity of 2-(2-methylpropoxy)ethanol to freshwater crustacean Daphnia magna was determined in a GLP compliant study in accordance with OECD 202 test guideline. Daphnia magna were exposed to the substance at concentrations of 100, 320, 1000, 3200 and 10000 mg/L. The 48h EC50 and 48h NOEC were 2100 mg/L and 1000 mg/L respectively. As the 48-hour EC50 is greater than 1 mg/L, the registered substance is not considered to be a acutely toxic to aquatic invertebrates (CLP Regulation (EC) No 1272/2008).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
2 100 mg/L

Additional information