N-dodecyl-β-alanine, compound with 2,2',2''-nitrilotriethanol (1:1)

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:

biodegradation in water: simulation testing on ultimate degradation in surface water

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because the substance is readily biodegradable

Cross-reference

Reason / purpose for cross-reference:

data waiving: supporting information

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

Study performed on sodium salt.
The triethanolamine salt also dissociates and triethanolamine biodegradation has been well investigated

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

28 d

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (CO2 evolution)

Value:

ca. 70

Sampling time:

9 d

Parameter:

% degradation (CO2 evolution)

Value:

ca. 96

Sampling time:

28 d

Details on results:

Two concentrations tested, but lower concentration (approx 10 mg/l C) gave a % degradation over over 100% and has been disregarded.
The higher concentration of approximately 30 mg/l C resulted in a plateau of about 100% degradation after day 22. The 10 day window was achieved.
The positive control group reached a plateau of about 80% after 22 days, but failed to reach the 60% in 10 day criteria.

Results with reference substance:

The positive control group reached a plateau of about 80% after 22 days, but failed to reach the 60% in 10 day criteria.

Validity criteria fulfilled:

no

Interpretation of results:

readily biodegradable

Executive summary:

There are obvious flaws in the testing, such as exceeding 100% for one of the concentrations tested and the poor performance of the positive control. However, earlier studies (albeit with less detail on methods and limited reporting) and estimations based on modelling and considering the class of substance suggests that the the substance is readily biodegradable.