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EC number: 276-339-5 | CAS number: 72102-40-0
Quality criteria of the test are satisfied. The mean amount of TIC present in the blank controls at the end of the test is <3 mg C/L. The biodegradation percentage of the test substance during the test is summarized in the following table.The trend of the inorganic carbon and related biodegradation percentages (See table below - column % of biodegradation Reference substance + test substance) in the bottle contained both the test substance and the reference substance confirm the absence of inhibitory effect of the test substance on inoculum.
% Of Biodegradation of Reference Substance
% Of Biodegradation of Test Substance
% Of Biodegradation of Reference Substance+ Test Substance
A study was conducted to determine the ready biodegradability of the test substance, 'iso and anteiso C10-40 AAP EDM-ES' (active: 96%), according to the OECD Guideline 310, Ready Biodegradability – CO2 in sealed vessels (Headspace Test) method, in compliance with GLP. Aerobic sludge inoculum was prepared with mixed treatment plant of urban (66%) and industrial (34%) liquid sewage and in order to have a concentration of suspended solid of about 4 mg/L. In the study, for inoculum blanks, positive control (reference substance: sodium benzoate at 23.66 mg/L) and test substance (23.39 mg/L) groups, 29 inoculum bottles replica each, whereas for toxicity control (test and reference substances) group, 11 inoculum bottles replica were used. All treated samples were kept at the temperature of 20°C for 56 d. The calculation of biodegradation as total inorganic carbon (TIC) were determined at each sampling time (0, 1, 7, 14, 21, 28, 35, 42, 49 and 56 d) for the reference substance, test sample and blank. For toxicity control, calculation of biodegradability as total inorganic carbon (TIC) was determined on Day 1, 28 and 56. The trend of the inorganic carbon and related biodegradation percentages in the bottle contained both the test sample and the reference substance did confirm the absence of inhibitory effect of the test sample on inoculum. At 28 d, the biodegradability of the test substance was determined to be 2%. At 14 d biodegradability for reference substance was determined to be 80%. The mean amount of TIC present in the blank controls at the end of the test was <3 mg C/L. Based on the study results, the test has satisfied the all validity criteria. Under the study conditions, the test substance, 'iso and anteiso C10-40 AAPEDM-ES', was determined to be not readily biodegradable (Eurofins, 2018).
Based on the study results, the test substance, 'iso and anteiso C10-40 AAP EDM-ES' was determined to be not readily biodegradable.
[Type of water: freshwater]
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