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EC number: 309-304-0 | CAS number: 100208-97-7
An acute oral toxicity study according to OECD guideline 423 was performed. The test item was administered oral via garvage. The day before treatment the animals were fasted. Food but not water was withheld overnight. Animals were weighed before the application and food was given back 3 hours after the treatment. The observation period was 14 days. The acute toxic class method was carried out involving a stepwise procedure with the use of 2000 mg dye/kg bw (corresponding to 2240 mg product/kg bw) as the starting dose in three female rats. Starting dose was selected on the basis of the available information about the test item. No animal died in the first step at 2000 mg/kg bw dose level, so treatment with 2000 mg/kg bw was repeated on further three female rats. No animal died in the second step, too, so the test was finished, the stopping criteria of Annex 2d of OECD Guideline No. 423 was met. The oral LD50 was determined to be > 2000 mg/kg bw.
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