2-Naphthalenesulfonic acid, 5(or 8)-amino-, reaction products with 4-aminophenol and sodium sulfide (Na2(Sx))

Endpoint summary

Description of key information

Short-term toxicity to invertebrates

In a Daphnia sp. Acute Immobilisation Test according to OECD Guideline 202 with a WAF, the test item Leuco Sulfur Blue 15 had no toxic effect up to aquatic saturation (i.e. limit test concentration); the 48h LOEC is higher than the solubility level of the test item in the test medium, which corresponds to a mean measured concentration of 0.50 mg/L (based on total product). The NOEC value is equal to the mean measured test item concentration of 0.50 mg/L (based on total product). The EC50 was determined to be > 0.5 mg/L.

 

Toxicity to aquatic plants other than algae

The influence of the test item on the growth of the freshwater aquatic plant Lemna gibba (duckweed) was investigated in a 7‑day static test, based on the OECD Guideline No. 221 “Lemna sp. Growth Inhibition Test” (2006), with nominal concentrations of 6.25, 12.5, 25, 50 and 100 mg test item/L. The test item Leuco Sulfur Blue 15 had no toxic effect at aquatic saturation on Lemna gibba; the EC10, EC50 and overall LOEC is higher than the nominal concentration of 100 mg/L of the test item in the test medium, which corresponds to the mean measured concentration of 1.69 mg/L, based on the water accommodated fraction of the test item.