trans-cyclohexane-1,2-dinitrilotetraacetic acid

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Justification for type of information:

The substance tetrasodium ethylenediaminetetraacetate (CAS 64-02-8) is structurally similar to the substance trans-cyclohexane-1,2-dinitrilotetraacetic acid (CAS 13291-61-7), therefore used for the read-across.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: EEC guideline 79/831/EEC, Annexe V, part C: Methods for the determination of ecotoxicity, algae: Test of growth inhibition, May 1988

GLP compliance:

yes

Analytical monitoring:

yes

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Test species: Scenedesmus subspicatus
Strain: CHODAT SAG 86.81
Source: Algae collection Goettingen, SAG 86.81

Test type:

static

Water media type:

freshwater

Limit test:

no

Test temperature:

23 ± 2 °C

pH:

7.65 - 8.80

Details on test conditions:

Aeration: none
No. of replicates:
- 3 inoculated replicates
- 1 uninoculated replicate for measuring the pH-value of the uninoculated test concentrations after an incubation time of 72 h.
- Controls: negative control

TEST MEDIUM / WATER PARAMETERS
- Dilution water: according to EEC guideline 79/831/EEC, Annexe V, part C:
- Methods for the determination of ecotoxicity, algae: Test of growth inhibition, May 1988 and the SOP of the laboratory
- Deviation from the SOP: Each test concentration was mixed according to the following regimen:
- 1 part of inoculum (1E+05 cells/ml in test medium)
- 1 part of test medium (10 times concentrated)
- 8 parts of test substance (in appropriate water dilutions)

TEST CONDITIONS
- Illumination: 120 µEinstein/m*m*s (permanent Illumination, Color of light was universal white, L 25 (Osram))
- Inoculation density: 10000 cells/mL (initial concentration in the test)

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L - 0.39 mg/L
- Stock solution: 125 mg/L

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.39 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

0.78 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.7 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

2.77 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

>= 100 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect on the development of biomass [mg/L]:

EbC 10 = 0.48 mg/L

EbC 50 = 1.01 mg/L

EbC 90 = 100 mg/L

 

Effect on growth rate [mg/L]:

ErC 10 = 0.70 mg/L

ErC 50 = 2.77 mg/L

ErC 90 => 100.0 mg/L

 

No observed effect concentration (95 % significance level):

NOEC (72 h) = 0.39 mg/L

 

Lowest observed effect concentration:

LOEC (72 h) = 0.78 mg/L

 

VALIDTY CRITERIA

The validity criteria were maintained. Factor of cell division (cell count) after 72 hours: 46

 

SUMMARY OF THE CONCENTRATION CONTROL ANALYSIS

The concentrations 100 and 12.5 mg/L, the untreated control and the stock solution (125 mg/L) were analysed. Test conditions and results are listed in the GLP-reports 94LOO650 and 94LOO663, dated 22 Nov 1994, from Dr. Scholtissek, BASF AG, ZAX/OF.

 

TIME SCAN

begin of test (0 h) - uninoculated replicate

end of test (72 h) - uninoculated replicate

end of test (72 h) - inoculated replicate after removal of algal cells through centrifugation

 

RECOVERY IN %:

                                  control  / test  / stock solution /

-----------------------------------------------------------

t= 0 h unoculated   <DL   / 100-104 /     99.2      /

t= 72 h unoculated  <DL   / 96-99 /     98.4      /

t= 72 h inoculated    <DL   / 99-104 /       -       /

-----------------------------------------------------------

DL= detection limit (1 mg/L)

Reference 2

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: EEC guideline 79/831/EEC, Annexe V, part C: Methods for the determination of ecotoxicity, algae: Test of growth inhibition, May 1988

GLP compliance:

yes

Analytical monitoring:

yes

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Test species: Scenedesmus subspicatus
Strain: CHODAT SAG 86.81
Source: Algae collection Goettingen, SAG 86.81

Test type:

static

Water media type:

freshwater

Limit test:

no

Test temperature:

23 ± 2 °C

pH:

7.65 - 8.80

Details on test conditions:

Aeration: none
No. of replicates:
- 3 inoculated replicates
- 1 uninoculated replicate for measuring the pH-value of the uninoculated test concentrations after an incubation time of 72 h.
- Controls: negative control

TEST MEDIUM / WATER PARAMETERS
- Dilution water: according to EEC guideline 79/831/EEC, Annexe V, part C:
- Methods for the determination of ecotoxicity, algae: Test of growth inhibition, May 1988 and the SOP of the laboratory
- Deviation from the SOP: Each test concentration was mixed according to the following regimen:
- 1 part of inoculum (1E+05 cells/ml in test medium)
- 1 part of test medium (10 times concentrated)
- 8 parts of test substance (in appropriate water dilutions)

TEST CONDITIONS
- Illumination: 120 µEinstein/m*m*s (permanent Illumination, Color of light was universal white, L 25 (Osram))
- Inoculation density: 10000 cells/mL (initial concentration in the test)

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L - 0.39 mg/L
- Stock solution: 125 mg/L

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.39 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

0.78 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.7 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

2.77 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

>= 100 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect on the development of biomass [mg/L]:

EbC 10 = 0.48 mg/L

EbC 50 = 1.01 mg/L

EbC 90 = 100 mg/L

 

Effect on growth rate [mg/L]:

ErC 10 = 0.70 mg/L

ErC 50 = 2.77 mg/L

ErC 90 => 100.0 mg/L

 

No observed effect concentration (95 % significance level):

NOEC (72 h) = 0.39 mg/L

 

Lowest observed effect concentration:

LOEC (72 h) = 0.78 mg/L

 

VALIDTY CRITERIA

The validity criteria were maintained. Factor of cell division (cell count) after 72 hours: 46

 

SUMMARY OF THE CONCENTRATION CONTROL ANALYSIS

The concentrations 100 and 12.5 mg/L, the untreated control and the stock solution (125 mg/L) were analysed. Test conditions and results are listed in the GLP-reports 94LOO650 and 94LOO663, dated 22 Nov 1994, from Dr. Scholtissek, BASF AG, ZAX/OF.

 

TIME SCAN

begin of test (0 h) - uninoculated replicate

end of test (72 h) - uninoculated replicate

end of test (72 h) - inoculated replicate after removal of algal cells through centrifugation

 

RECOVERY IN %:

                                  control  / test  / stock solution /

-----------------------------------------------------------

t= 0 h unoculated   <DL   / 100-104 /     99.2      /

t= 72 h unoculated  <DL   / 96-99 /     98.4      /

t= 72 h inoculated    <DL   / 99-104 /       -       /

-----------------------------------------------------------

DL= detection limit (1 mg/L)

Key value for chemical safety assessment

EC50 for freshwater algae:

2.77 mg/L

EC10 or NOEC for freshwater algae:

0.7 mg/L