Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990-06-12 (experimental phase)
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Version / remarks:
ISO-Vorschrift 8192-1986 (E)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron
EC Number:
256-526-8
EC Name:
[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron
Cas Number:
50293-39-5
Molecular formula:
C32H18FeN12
IUPAC Name:
[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron
Test material form:
solid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge
Details on inoculum:
- Name and location of sewage treatment plant where inoculum was collected: Laborkläranlage (OECD)
- Initial biomass concentration: 6 g/l dry matter

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Remarks on exposure duration:
not stated in report

Test conditions

Nominal and measured concentrations:
nominal 100, 1000, 1000 mg/l
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrations
Key result
Dose descriptor:
EC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks on result:
not determinable
Remarks:
no sufficient inhibition
Results with reference substance (positive control):
Test valid, positive control gave the appropriate results

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
positive control gave the appropriate result
Conclusions:
The study was conducted according to ISO-Vorschrift 8192-1986 (E) on the registered substance itself with minor deficiencies in documentation. However, the given data indicate that the study was well and scientifically reasonable performed. The positive control induced the appropriate response. Hence, the results can be considered as sufficiently reliable to assess the toxicity of the test item towards microorganisms. The determined EC50 was > 10000 mg/l, hence, the substance can be considered as non-toxic to activated sludge.
Executive summary:

The potential hazard of the test item towards microorganisms was tested in an ISO-Vorschrift 8192-1986 (E) study. Concentrations ranging from 100 to 10000 mg/l were applied to activated sludge and the respiration rate was determined. Concentrations up to 1000 mg/l did not result in an inhibition of the respiration rate, 10000 mg/l induced an inhibition of 25%. 3,5-dichlorophenol served as positive control and induced the appropriate results. The test item was non-toxic to activated sludge, the EC50 was determined as > 10000 mg/l.