Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-716-8 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No GLP, study according to guidance of German Environmental Agency
- Qualifier:
- according to guideline
- Guideline:
- ISO 10708 Water quality - Evaluation in an aqueous medium of the ultimate aerobic biodegradability of organic compounds - Determination of biochemical oxygen demand in a two-phase closed bottle test
- Version / remarks:
- 1990
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): aeration tank of the municipal sewage treatment plant of Pforzheim, Germany
- Preparation of inoculum for exposure: 2 L of mineral medium were inoculated with 20 mL of activated sludge (3.0 g/L). 200 mL of this solution was transferred into 9 test vessels with magnetic stirring bars. Bottles were closed with glass stoppers and incubated without test item for 1 week in the dark at 20 °C and 100 rpm.
- Pretreatment: activated sludge was washed twice by centrifugation (10 min., 6000 rpm) with mineral solution. Pellet was mixed with mineral solution (final sludge concentration of 3.0 g/L).
- Concentration of inoculum: 30 mg suspended solids per litre - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- ThOD/L
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: in 1 L: KH2PO4 85 mg, K2HPO4 217.5 mg, Na2HPO4*2H2O 334 mg, NH4Cl 5 mg, CaCl2*2H2O 36.42 mg, MgSO4*7H2O 22.5 mg, FeCl3*6H2O 0.25 mg
- Test temperature: 20 °C
- pH: blank: 7.38 - 7.49, reference control: 6.87 - 7.62, test item: 7.36 - 7.46
- Aeration of dilution water: 15 min prior to test start
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: closed glass bottles with 2/3 aqueous test mixture and 1/3 air
- Number of culture flasks/concentration: 3
- Measuring equipment: oximeter (WTW Oxi 191)
- Other: continuous shaking 100 rpm
SAMPLING
- Sampling frequency: 8, 14, 22, and 28 d alter the start of the experiment
- Sampling method: the remaining O2 concentration in the test vessels was measured
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 replicates
- Reference control: 3 replicates - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 12.43
- Sampling time:
- 28 d
- Results with reference substance:
- 67.33% degradation after 28 d (more than 60% after 14 d).
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable under test conditions
- Conclusions:
- The test item was not readily biodegradable (12.43% based on O2 consumption) under the chosen test conditions.
- Executive summary:
There is one study available, in which the ready biodegradability of test item was assessed according to the BODIS test guidance for insoluble substances of the German Environmental Agency (1990).
The test item at a concentration of 20 mg ThOD was inoculated with 30 mg/L of a mixed bacterial inoculum in closed glass bottles with 2/3 of aqueous test mixture and 1/3 headspace for 28 d. The degradation process was followed by weekly measurements of the BOD in the aqueous phase. Degradation was expressed as BOD to ThOD in %. After 28 d, the average degradation rate of the test item was 12.43%. The degradation of the reference substance sodium benzoate reached 67.33% after 28 d, thus confirming the suitability of the inoculum.
Reference
Total oxygen uptake in the blanks after 1 week was < 3 mg O2/L and in the following weeks not >= l mg/L per week.
Table 1: Cumulative degradation at day 28.
| Sample | Cumul. Degradation (%) |
| 28 d | |
| Blank 1 | - |
| Blank 2 | - |
| Blank 3 | - |
| Reference 1 | 72.089 |
| Reference 2 | 62.096 |
| Reference 3 | 67.803 |
| Test item 1 | 11.099 |
| Test item 2 | 11.68 |
| Test item 3 | 14.496 |
Description of key information
Not readily biodegradable (12.43% within 28 d, BODIS test)
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
There is one study available, in which the ready biodegradability of test item was assessed according to the BODIS test guidance for insoluble substances of the German Environmental Agency (1990).
The test item at a concentration of 20 mg ThOD was inoculated with 30 mg/L of a mixed bacterial inoculum in closed glass bottles with 2/3 of aqueous test mixture and 1/3 headspace for 28 d. The degradation process was followed by weekly measurements of the BOD in the aqueous phase. Degradation was expressed as BOD to ThOD in %. After 28 d, the average degradation rate of the test item was 12.43%. The degradation of the reference substance sodium benzoate reached 67.33% after 28 d, thus confirming the suitability of the inoculum.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
