Registration Dossier
Registration Dossier
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EC number: 816-285-7 | CAS number: 1263133-33-0
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
- Deviations:
- yes
- Remarks:
- Refer section 'Any other information on materials and methods incl.tables'
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1400 (Fish Early-life Stage Toxicity Test)
- Deviations:
- yes
- Remarks:
- Refer section 'Any other information on materials and methods incl.tables'
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Remarks:
- UV-sterilized, filtered well water
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 88 d
- Hardness:
- 140 to 144 mg/L (as CaCo3)
- Test temperature:
- 11.5 to 12.8°C
- pH:
- 7.9 to 8.2
- Dissolved oxygen:
- ≥ 6.9 mg/L (≥64% of saturation)
- Conductivity:
- 284 to 365 μmhos/cm
- Nominal and measured concentrations:
- Nominal: 0.63, 1.3, 2.5, 5.0 and 10 mg a.s./L
Measured: 0.60, 1.3, 2.4, 4.9 and 9.7 mg a.s./L - Key result
- Duration:
- 88 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 9.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Hatching success, time to hatch, time for larvae to swim-up, post-hatch survival or post-hatch growth (total length, wet and dry weights)
- Key result
- Duration:
- 88 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 9.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Hatching success, time to hatch, time for larvae to swim-up, post-hatch survival or post-hatch growth (total length, wet and dry weights)
- Key result
- Duration:
- 88 d
- Dose descriptor:
- other: Maximum Acceptable Toxicant Concentration
- Effect conc.:
- > 9.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Validity criteria fulfilled:
- yes
- Conclusions:
- 88 Day NOEC (Rainbow Trout): 9.7 mg a.s./L (Highest concentration tested) (Hatching success, time to hatch, time for larvae to swim-up, post-hatch survival or post-hatch growth (total length, wet and dry weights).
88 Day LOEC (Rainbow Trout): >9.7 mg a.s./L (Highest concentration tested) (Hatching success, time to hatch, time for larvae to swim-up, post-hatch survival or post-hatch growth (total length, wet and dry weights).
88 Day MATC (Rainbow Trout): >9.7 mg a.s./L (Highest concentration tested) (Hatching success, time to hatch, time for larvae to swim-up, post-hatch survival or post-hatch growth (total length, wet and dry weights). - Executive summary:
The Study was conducted according to guidelines OECD Guideline 210 and US EPA OPPTS 850.1400 to determine the effects of test substance on the early life stages (based on time to hatch, hatching success, time to swim-up, survival and growth) of rainbow trout (Oncorhynchus mykiss) in an unaerated flow-through system for 88 days.
Treatments consisted of a dilution water control, solvent control (0.1 mL dimethylformamide/L) and five nominal test substance concentrations of 0.63, 1.3, 2.5, 5.0 and 10 mg a.s./L. The corresponding mean, measured concentrations of test substance were 0.60, 1.3, 2.4, 4.9 and 9.7 mg a.s./L. The observations showed that there were no treatment-related effects on hatching success, time to hatch, time for larvae to swim-up, post-hatch survival or post-hatch growth (total length, wet and dry weights).
Under the conditions of the test, the 88-day NOEC and LOEC for rainbow trout based on mean, measured concentrations of test substance and hatching success, time to hatch, time for larvae to swim-up, post-hatch survival or post-hatch growth (total length, wet and dry weights) were 9.7 mg a.s./L and >9.7 mg a.s./L, respectively. The MATC was calculated to be >9.7 mg a.s./L.
Reference
Description of key information
88-Day NOEC (Oncorhynchus mykiss, Rainbow trout) = 9.7 mg/L:OECD 210; Reliability = 1
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 9.7 mg/L
Additional information
The 88-day NOEC for rainbow trout, in the OECD guideline 210 test, based on mean, measured concentrations of test substance and hatching success, time to hatch, time for larvae to swim-up, post-hatch survival or post-hatch growth (total length, wet and dry weights) was 9.7 mg a.s./L.
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