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Short-term toxicity to fish

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The acute toxicity of Witconate P-1460 to the fresh water fish Dania rerio (zebra fish) was tested under static conditions for 96 hours in accordance with OECD Guidelines for testing of chemicals, 203: Fish, acute toxicity test (7/1992) and EEC Directive 79/831, Annex V, part C: Methods for the determination of ecotoxicity, part C.1. Acute toxicity to fish (update 11/1989) The test was performed in compliance with Good Laboratory Practice (GLP).

Witconate P-1460 is miscible in water. A preliminary test on Dania rerio, performed as a non-GLP test, showed no lethality at 1 mg/L but 2 out of the three fish were lying on the bottom after 96 hours of testing. At 10 mg/L 100 % death was observed after 24 hours. At 100 mg/L 100% death was observed after 4 hours.The results of the definitive test show that the test substance had 100% lethal effect at 9.37 mg/L of Witconate P-1460 after 24 hours of testing, but no lethal or sub-lethal effects were observed at the other test concentrations of respectively 0.4, 0.88 1.94 and 4.26 mg/L.

Based on the test results a NOEC (no observed effect concentration) and the LOEC (lowest observed effect concentration) (0-96h) of Witconate P-1460 was derived of 4.26 mg/Land 9.37 mg/L respectively. Based on the available data an LC50 was calculated of 6.32 mg/L. The LC100 observed after 96 hours of testing 9.37 mg/L Witconate P-1460. No other sub-lethal effects were observed in the tested concentrations.

The results of the chemical analyses show that the average observed test substance concentrations during the test differed less than 20% from the nominal test concentrations. The results are therefore presented based on their nominal concentrations. The average observed test substance concentrations were found to be all higher than the nominal concentrations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
6.32 mg/L

Additional information