3-amino-N,N-dimethylpropan-1-aminium 2-C10-13-alkyl benzenesulfonate

Endpoint summary

Description of key information

The acute toxicity of Witconate P-1460 to the fresh water fish Dania rerio (zebra fish) was tested under static conditions for 96 hours in accordance with OECD Guidelines for testing of chemicals, 203: Fish, acute toxicity test (7/1992) and EEC Directive 79/831, Annex V, part C: Methods for the determination of ecotoxicity, part C.1. Acute toxicity to fish (update 11/1989) The test was performed in compliance with Good Laboratory Practice (GLP).

Witconate P-1460 is miscible in water. A preliminary test on Dania rerio, performed as a non-GLP test, showed no lethality at 1 mg/L but 2 out of the three fish were lying on the bottom after 96 hours of testing. At 10 mg/L 100 % death was observed after 24 hours. At 100 mg/L 100% death was observed after 4 hours.The results of the definitive test show that the test substance had 100% lethal effect at 9.37 mg/L of Witconate P-1460 after 24 hours of testing, but no lethal or sub-lethal effects were observed at the other test concentrations of respectively 0.4, 0.88 1.94 and 4.26 mg/L.

Based on the test results a NOEC (no observed effect concentration) and the LOEC (lowest observed effect concentration) (0-96h) of Witconate P-1460 was derived of 4.26 mg/Land 9.37 mg/L respectively. Based on the available data an LC50 was calculated of 6.32 mg/L. The LC100 observed after 96 hours of testing 9.37 mg/L Witconate P-1460. No other sub-lethal effects were observed in the tested concentrations.

The results of the chemical analyses show that the average observed test substance concentrations during the test differed less than 20% from the nominal test concentrations. The results are therefore presented based on their nominal concentrations. The average observed test substance concentrations were found to be all higher than the nominal concentrations.

The acute toxicity of Witconate P-1460 to Daphnia magna (water flea) was tested under static conditions for 48 hours in accordance with OECD 202 and EEC C.2 guidelines for testing chemicals, and ECETOC, Monograph 26 (1996). The test was performed in compliance with Good Laboratory Practice (GLP).

Witconate P-1460 is miscible in water. A preliminary test showed that Witconate P-1460 had a 100% toxicity effect at a test concentration 10 mg/L and an observed NOEC of 1 mg/L having slightly sub lethal effects. Therefore a toxicity test was performed exposing D. magna to a series of test concentrations respectively 0.5, 1.1, 2.3, 4.9 and 10.2 mg/L of test substance.

The results of the definitive test show that the test substance had effect on the mobility of D. magna.

The NOEC and the LOEC (0-48h) of Witconate P-1460 are 2.3 mg/L and 4.9 mg/L respectively. EC50 and EC100 values of respectively 3.6 mg/L (3.3 - 4.0, 95% confidence limits) and 10.2 mg/L were determined. No further sub-lethal effects were observed in the test concentrations where no immobility was observed. The results of the chemical analyses show that the average observed test substance concentrations during the test differs less than 20% from the nominal test concentrations. The results are therefore presented based on their nominal test concentrations.

In order to predict effects of chemicals in an aquatic environment, the toxicity to freshwater algae was assessed. The algal toxicity was determined in the Algal Growth Inhibition test in accordance with OECD 201, EEC and ISO 8692 test guidelines and with the OECD Principles of Good Laboratory Practice. The guidelines were slightly modified to ensure good growth and pH control of the cultures.

The green algae Pseudokirchneriella subcapitata (formerly known as Se/enastrum capricomutum) was exposed to the following nominal concentrations: 0, 4.3, 9.4, 20.7, 45.5 and 100 mg/L prepared from the 0.1 g/L stock solution of Witconate P-1460. The toxicity of Witconate P-1460 to an exponentially growing culture of P. subcapitata was determined over an exposure period of 72 hours. The EbC50 and ErC50 (0 - 72 h) are 46.3 (95% confidence limits: 26.4 - 89.1) and >100 mg/L, respectively. The NOEC and LOEC are 9.4 mg/I and 20.7 mg/I, respectively. The test was conducted in a mineral salts medium in a climatized illuminated orbital incubator. The maximum variation in pH in the test media was 1.0 pH unit.

The definitive test is valid as shown by the increase of the extinction of the control over 72 h by a factor of 134.

The results of the chemical analyses show that the initial concentrations in the test solutions are close to the nominal (expected) concentrations. A decrease of >70% of test substance concentration during the 72 hours of testing is observed at 4.6 and 20.7 mg/L. No decrease is observed at 100 mg/L. Degradation and sorption to glassware of the test substance are both unlikely reasons for the decrease because at 100 mg/L no significant decrease of the test concentration is observed. For chemical analyses, the samples were filtered to remove the algae. The most obvious reason for the observed decrease is therefore adsorption of the test substance to the algae. If no degradation or sorption to glassware occurred, the algae in the test vessels were fully exposed to the test substance. Therefore the nominal test concentrations were used to calculate the effects.