Nonane-1,9-diol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003-10-13 to 2004-10-15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Version: 4. April 1984

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Version / remarks:

Public Draft, April 1996

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

December 1992

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Batch no. 42638; Substance number: 03/0183-1

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: ambient, under N2
- Stability under test conditions: The stability of the test substance under storage conditions over the test period was guaranteed by analysis.

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Before weight in the test substance was homogenised 1 h at 80°C under Ihe test N2 in a water bath.

FORM AS APPLIED IN THE TEST (if different from that of starting material): liquid

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 100, 50, 25 and 12.5 mg/L
- Sampling method: At the begin (after 0 h) samples from vessels without daphnids and at the end of the exposition (after 48 h) samples from vessels with daphnids were analysed.
- Sample storage conditions before analysis: None (analyzed immediately after sample delivery).

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was stirred in M4 medium for about 10 minutes at approx. 60°C and for about 10 minutes at approx. 20°C ± 2°C. The concentration of the stock solution was 100.3 mg/L.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): The stock solution was colouriess/clear.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: STRAUS 1820
- Source: Institut National de Recherche Chimique Appliquee, France
- Age of parental stock (mean and range, SD): 2 - 4 weeks
- Age of the animals at the begin of exposition: 2 - 24 h (starting with the 3rd breed of parent animals)
- Feeding during test: None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.43 mmol/L

Test temperature:

20.2 - 20.9°C
Continuously measured during the whole test period in a separate vessel close to the test vessels.

pH:

8 - 8.1
After 0 h in an additional replicate without daphnids and after 48h in replicate 1 of each tested concentration.

Dissolved oxygen:

8.0 - 8.9 mg/L
After 0 h in an additional replicate without daphnids and after 48h in replicate 1 of each tested concentration.

Nominal and measured concentrations:

Nominal: 0, 100, 50, 25 and 12.5 mg/L
Measured: The analytical recovery rates in the range of the EC-values were > 80%. Therefore no transformation of the nominal EC-values into the values of the concentration control analysis occurs.

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 mL)
- Material, size, headspace, fill volume: Material: glass; Fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water was used for culture and test purposes. For the composition of this M4 medium see ISO 10706.
- Alkalinity up to pH 4.3: 0.86 mmol/L
- Ca/mg ratio: about 4 : 1
- Conductivity: 612 µS/cm

OTHER TEST CONDITIONS
- Photoperiod: day:night rhythm = 16 : 8 h
- Light intensity: About 1 - 8 µE/(m2*s) at a wave length of 400 - 750 nm, Artificial light, type warm white (OSRAM L58 W31)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Swimming ability of the test animals (Animals which were unable to swim within 15 seconds, even after gentle agitation of the test vessel were considered to be immobile), visually after 0, 24 and 48 h

VEHICLE CONTROL PERFORMED: no

RANGE-FINDING STUDY
- Results used to determine the conditions for the definitive study: The test concentrations were determined in a range finding test (non GLP).

Reference substance (positive control):

yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50 (24h): 1.44 mg/L

Table 1: Number of mobile Daphnids in the different test concentrations.

Nominal conc. [mg/L]	Parallel 1 [h]			Parallel 2 [h]			Parallel 3 [h]			Parallel 4 [h]			Sum [h]	Sum [h]
	0	24	48	0	24	48	0	24	48	0	24	48	24	48
Control	5	5	5	5	5	5	5	5	5	5	5	5	20	20
100	5	5	5	5	5	3	5	5	4	5	5	3	20	15
50	5	5	5	5	5	5	5	5	5	5	5	5	20	20
25	5	5	5	5	5	5	5	5	5	5	5	5	20	20
12.5	5	5	5	5	5	5	5	5	5	5	5	5	20	20

 

Table 2: Analytically measured relative minimum and maximum concentrations of the test substance in the test solutions. The values are given in percent of the nominal concentrations.

Time [h]	Stocked with daphnids	Minimum [%]	Maximum [%]
0	No	59.5	84.7
48	Yes	67.8	89.8

 

Table 3: pH- and oxygen values in the test solutions after 0 h and 48 h

Nominal concentration [mg/L]	pH value after 0 h *	pH value after 48 h **	O2value [mg/L] after 0 h *	O2value [mg/L] after 48 h *
Control	8.1	8.1	8.6	8.9
100	8.0	8.0	8.0	8.5
50	8.0	8.1	8.5	8.6
25	8.0	8.1	8.6	8.7
12.5	8.1	8.1	8.5	8.8

* measured in the additional replicate 5 of each concentration

** measured in the replicate 1 of each concentration

Validity criteria fulfilled:

yes

Conclusions:

The EC50 (48 hours) was determined with > 100 mg/L.

Executive summary:

The toxicity of the test substance on Daphnia magna was determined according to OECD 202 and in compliance with GLP. Individuals of D. magna were exposed to the substance in concentrations of 0, 12.5, 25, 50 and 100 mg/L for 48 hours. Concentration were analytically verified by GC-FID. As the measured concentrations were > 80 % of the nominal, effects values are based on nominal concentrations. The EC50 (48 hours) was determined with > 100 mg/L.

Description of key information

EC50 (48 h) > 100 mg/L (nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The toxicity of the test substance on Daphnia magna was determined according to OECD 202 and in compliance with GLP. Individuals of D. magna were exposed to the substance in concentrations of 0, 12.5, 25, 50 and 100 mg/L for 48 hours. Concentration were analytically verified by GC-FID. As the measured concentrations were > 80 % of the nominal, effects values are based on nominal concentrations. The EC50 (48 hours) was determined with > 100 mg/L.