Registration Dossier

Administrative data

Description of key information

These endpoints were fulfilled using read across from 3,7-dimethyloct-6-enenitrile (EC 257-288-8 / CAS 51566-62-2), for which the following results were obtained.

Acute toxicity: Oral :

Tested doses: 3.15, 3.96, 4.46, 6.30 and 7.94 g/kg

LD50 = 4.49 (3.74 - 5.39) g/kg

Acute toxicity: Inhalation:

The acute toxicity via the inhalation route was assessed using test guideline equivalent or similar to OECD 403. LC50: >4.9 mg/L on male / females.

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Referenceopen allclose all

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
November 9, 1979 - December 5, 1979
Reliability:
2 (reliable with restrictions)
Reason / purpose:
reference to same study
Qualifier:
no guideline available
Principles of method if other than guideline:
Study carried out in 1979.
GLP compliance:
yes
Limit test:
no
Species:
rat
Strain:
Wistar
Remarks:
albino
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Age at study initiation: approximately 6 to 8 weeks of age
- Weight at study initiation: 200 - 266 g
- Fasting period before study: 18 hours of fasting
- Housing: galvanized cages with indirect bedding
- Diet: ad libitum.
- Water: ad libitum.
- Acclimation period: at least 2 days

ENVIRONMENTAL CONDITIONS
- Temperature: controlled
- Photoperiod: 12 hour lightldark cycle
Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Doses:
3.15, 3.96, 4.46, 6.30 and 7.94 g/kg
No. of animals per sex per dose:
3
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: 1, 3, 6, and 24 hours after treatment, and daily thereafter for a total of 14 days.
- Necropsy of survivors performed: yes
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
4.49 other: g/kg
Based on:
test mat.
Mortality:
See attachment
3.15 g/kg: 16.7%
3.96 g/kg: 33.3%
4.46 g/kg: 83.3%
6.30 g/kg: 83.3%
7.94 g/kg: 83.3%
Clinical signs:
See attachment
Body weight:
See attachment
Gross pathology:
See attachment
3.15 g/kg: Animal #la: Fibrous tissue encasing heart and lungs (died on day 11). #2,#3,#4a,#5,#6: No gross changes observed.
3.96 g/kg: Animal #1: Fibrous tissue encasing heart and lungs. #2,#5,#6: No gross changes observed. #3: No gross changes observed. #4: Head partially cannibalized. Pyloric mucosa severely reddened. Stomach ruptured. All abdominal viscera adhered to body wall and covered with a thin layer of fibrous tissue.
4.46 g/kg: Animal #1,#2: No gross changes observed. #3: Liver extremely pale. Intestines ruptured. #4,#6: Partially cannibalized. No gross changes observed.
#5a: No gross changes observed.
6.30 g/kg: Animal #1,#2,#6: Moderately reddened pyloric mucosa. #3a: Test article in stomach. No gross changes observed. #4: No gross changes observed. #5: No gross changes observed.
7.94 g/kg: Animal #1: No gross changes observed. a #2: Partially cannibalized. No gross changes observed. #3: No gross changes observed.#4-#6: No gross changes observed.
Other findings:
See attachment
Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
LD50 = 4.49 (3.74 - 5.39) g/kg
Endpoint:
acute toxicity: oral
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
November 9, 1979 - December 5, 1979
Reliability:
2 (reliable with restrictions)
Justification for type of information:
ANALOGUE APPROACH

1. HYPOTHESIS FOR THE ANALOGUE APPROACH
The structures of the target and source substances are identical and differ only with respect to the ratio of enantiomers where the target substance is a single pure L-isomer and the source substance is an equimolar mixture of L and D isomers.

2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
The target substance, L-Citronellyl nitrile, is a mono-constituent substance (EC No. 695-909-8, CAS no. 35931-93-2).
The source substance, DL-Citronellyl nitrile, is a mono-constituent substance (EC No. 257-288-8, CAS no. 51566-62-2).
The source and target substances are both of high purity with a low concentration of impurities.

3. ANALOGUE APPROACH JUSTIFICATION
The read across hypothesis is based on structural similarity where the only difference between target and source molecules is the enantiomeric ratio. In a non-chiral environment the target and source chemicals will have identical properties but in the chiral environment of living organisms the enantiomers may possess different carcinogenicity and teratogenicity (in a chiral environment, stereoisomers might experience selective absorption, protein binding, transport, enzyme interactions and metabolism, receptor interactions, and DNA binding). Therefore, as a precaution for the developmental toxicity endpoint it is suggested that the NOAEL 250 mg/kg bw/day for L-Citronellyl nitrile is used instead of 500 mg/kg bw/day, as it is not known which form is more potent in vivo. All other endpoints are considered to be acceptable for this substance assuming that 50% of the target compound is available in the test material.

4. DATA MATRIX
Please refer to the data matrix included in the read-across justification document attached in Section 13.2.
Reason / purpose:
read-across source
Qualifier:
no guideline available
Principles of method if other than guideline:
Study carried out in 1979.
GLP compliance:
yes
Limit test:
no
Species:
rat
Strain:
Wistar
Remarks:
albino
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Age at study initiation: approximately 6 to 8 weeks of age
- Weight at study initiation: 200 - 266 g
- Fasting period before study: 18 hours of fasting
- Housing: galvanized cages with indirect bedding
- Diet: ad libitum.
- Water: ad libitum.
- Acclimation period: at least 2 days

ENVIRONMENTAL CONDITIONS
- Temperature: controlled
- Photoperiod: 12 hour lightldark cycle
Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Doses:
3.15, 3.96, 4.46, 6.30 and 7.94 g/kg
No. of animals per sex per dose:
3
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: 1, 3, 6, and 24 hours after treatment, and daily thereafter for a total of 14 days.
- Necropsy of survivors performed: yes
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
4.49 other: g/kg
Based on:
test mat.
Mortality:
See attachment
3.15 g/kg: 16.7%
3.96 g/kg: 33.3%
4.46 g/kg: 83.3%
6.30 g/kg: 83.3%
7.94 g/kg: 83.3%
Clinical signs:
See attachment
Body weight:
See attachment
Gross pathology:
See attachment
3.15 g/kg: Animal #la: Fibrous tissue encasing heart and lungs (died on day 11). #2,#3,#4a,#5,#6: No gross changes observed.
3.96 g/kg: Animal #1: Fibrous tissue encasing heart and lungs. #2,#5,#6: No gross changes observed. #3: No gross changes observed. #4: Head partially cannibalized. Pyloric mucosa severely reddened. Stomach ruptured. All abdominal viscera adhered to body wall and covered with a thin layer of fibrous tissue.
4.46 g/kg: Animal #1,#2: No gross changes observed. #3: Liver extremely pale. Intestines ruptured. #4,#6: Partially cannibalized. No gross changes observed.
#5a: No gross changes observed.
6.30 g/kg: Animal #1,#2,#6: Moderately reddened pyloric mucosa. #3a: Test article in stomach. No gross changes observed. #4: No gross changes observed. #5: No gross changes observed.
7.94 g/kg: Animal #1: No gross changes observed. a #2: Partially cannibalized. No gross changes observed. #3: No gross changes observed.#4-#6: No gross changes observed.
Other findings:
See attachment
Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
LD50 = 4.49 (3.74 - 5.39) g/kg
Endpoint conclusion
Endpoint conclusion:
adverse effect observed
Dose descriptor:
LD50
Value:
4 490 mg/kg bw
Quality of whole database:
Read across from 3,7-dimethyloct-6-enenitrile (EC 257-288-8)

Acute toxicity: via inhalation route

Link to relevant study records

Referenceopen allclose all

Endpoint:
acute toxicity: inhalation
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP compliant non-guideline study, available as unpublished report, no restrictions, fully adequate for assessment.
Justification for type of information:
ANALOGUE APPROACH

1. HYPOTHESIS FOR THE ANALOGUE APPROACH
The structures of the target and source substances are identical and differ only with respect to the ratio of enantiomers where the target substance is a single pure L-isomer and the source substance is an equimolar mixture of L and D isomers.

2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
The target substance, L-Citronellyl nitrile, is a mono-constituent substance (EC No. 695-909-8, CAS no. 35931-93-2).
The source substance, DL-Citronellyl nitrile, is a mono-constituent substance (EC No. 257-288-8, CAS no. 51566-62-2).
The source and target substances are both of high purity with a low concentration of impurities.

3. ANALOGUE APPROACH JUSTIFICATION
The read across hypothesis is based on structural similarity where the only difference between target and source molecules is the enantiomeric ratio. In a non-chiral environment the target and source chemicals will have identical properties but in the chiral environment of living organisms the enantiomers may possess different carcinogenicity and teratogenicity (in a chiral environment, stereoisomers might experience selective absorption, protein binding, transport, enzyme interactions and metabolism, receptor interactions, and DNA binding). Therefore, as a precaution for the developmental toxicity endpoint it is suggested that the NOAEL 250 mg/kg bw/day for L-Citronellyl nitrile is used instead of 500 mg/kg bw/day, as it is not known which form is more potent in vivo. All other endpoints are considered to be acceptable for this substance assuming that 50% of the target compound is available in the test material.

4. DATA MATRIX
Please refer to the data matrix included in the read-across justification document attached in Section 13.2.
Reason / purpose:
read-across source
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Principles of method if other than guideline:
BASF-Test: The study was conducted according to an internal BASF method which in principle is comparable to the OECD Guideline 403.
A test group consisting of 10 animals/sex was treated by single inhalation application; aerosol of the test substance. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 14 days, the surviving animals were sacrificed for the purpose of necropsy.
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes
Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Dr. K. Thomae GmbH, D-7950 Biberach, FRG
- Age at study initiation: 8 weeks
- Weight at study initiation: male animals 256 ± 22 g, female animals 176 ± 13 g.
- Housing: They were housed in groups of five in wire cages of Becker, type D III, without bedding
- Diet: The animais were offered maintenance diet for rats, mice and hamsters, SSNIFF R 10 mm pellet, SSNIFF-Versuchstierdiaten GmbH, D-4770 Soest, FRG.
- Water: Tap water ad libitum during the post-exposure observation period.

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12/12
Route of administration:
inhalation: aerosol
Type of inhalation exposure:
nose/head only
Vehicle:
air
Details on inhalation exposure:
TECHNICAL PROCEDURE:
- Exposure system: Head-nose inhalation system INA 20 (glass-steel construction, BASF Aktiengesellschaft, V ≈ 55 L); the animals are restrained in tubes and their snouts project into the inhalation chamber.
- Generator system: By means of a continuous infusion pump, INFU 362 (INDIGEL/Switzerland) and a two-component atomizer, mod. 970 (Schlick), a mixture of aerosol and air was generated.
- Experimental procedure: Constant amounts of the substance to be tested were supplied to a two-component atomizer by means of a metering pump. By means of compressed air (1.45 bar) an aerosol was generated, which was passed into the inhalation system. The exhaust air system was adjusted by 10% lower against the supply air system (positive pressure). This ensured that the mixture of test substance and air was not diluted by laboratory air in the breathing zones of the animals.

ANALYTICAL INVESTIGATIONS:
- Sampling: Apparatus: Quartz wool and a downstream fritted glass flask, BASF sampling station (with gas meter, impulse counter and automatic pump switching); Sorption solvent: 2-propanol; Sampling velocity: 1.25 m/s; Sampling amount: 1 L; Sampling site: immediately adjacent to the animal noses; Sampling probe ø: 7 mm; Sampling frequency: 1 sample every hour.
- Analytical method of determination: A gas-chromatographic method was used for the quantitative assay of the aerosol concentration. The aerosol samples were taken up in 40 mL 2-propanol (solvent). Gas-chromatographic method: GC HP/5840 A (Hewlett Packard). An internal standard was pipetted to the absorption sample contained in a 50 mL measuring flask and made up to the calibration mark. The chromatograpbic peak area was compared with calibration values and calculated.
- Particle size analysis: Equipment: Andersen Stack Sampler Mark III, Millipore vacuum compressed air pump XX 60 220 50, limiting orifice 3 L/min Millipore, sampling probe, internal diameter 6.9 mm, stopwatch. Procedure: 30 minutes after the beginning of the test at the earliest, one sample of the test group was taken for the particle size analysis. Before the sampling, the impactor was equipped with metal collecting discs and a backup particle filter. The impactor was connected to the pump and the test apparatus, and a sample (12 L) was removed. The impactor was taken apart, the collecting discs and the pre-impactor were rinsed with the solvent, the backup particle filter was eluted, and the samples obtained were analyzed by gas chromatography.
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
4.9 mg/L
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
Observation period: After the exposure period the surviving animals were observed for 14 days.
Clinical examinations: The body weight of the animals was checked before the beginning of the test, after 7 days and at the end of the observation period and was presented graphically. Clinical symptoms were recorded each workday. Mortality was checked each day.
Pathology: At the end of the 14-day observation period, the animals were sacrificed by CO2 and were subjected to a grosspathological examination.
Statistics:
The statistical evaluation of the concentration-response relationship was carried out in accordance with the binomial test (Wittig, H.: Mathematische Statistik 1974, pp. 32 - 35) according to tables of the BASF Computer Center.
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 4.9 mg/L air
Based on:
test mat.
Exp. duration:
4 h
Mortality:
No mortality was observed.
Clinical signs:
other: During exposure: aqueous discharge from noses, salivation. After exposure: aqueous, slight discharge from noses, salivation, reddened ears and limbs. After one day the animals were without findings.
Body weight:
The body weights of the test group were unimpaired on comparison to a control collective.
Gross pathology:
Organs: no abnormalities detected.
Other findings:
The MMAD 50% = 3.0 µm (geometrical standard deviation 2.9) was calculated from the results of the particle size analysis.
A fraction of 89% of the aerosol passing through the alveoli was obtained from the results of the particle size analysis.
Interpretation of results:
GHS criteria not met
Conclusions:
LC50: >4.9 mg/L on male / females.
Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP compliant non-guideline study, available as unpublished report, no restrictions, fully adequate for assessment.
Reason / purpose:
reference to same study
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Principles of method if other than guideline:
BASF-Test: The study was conducted according to an internal BASF method which in principle is comparable to the OECD Guideline 403.
A test group consisting of 10 animals/sex was treated by single inhalation application; aerosol of the test substance. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 14 days, the surviving animals were sacrificed for the purpose of necropsy.
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes
Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Dr. K. Thomae GmbH, D-7950 Biberach, FRG
- Age at study initiation: 8 weeks
- Weight at study initiation: male animals 256 ± 22 g, female animals 176 ± 13 g.
- Housing: They were housed in groups of five in wire cages of Becker, type D III, without bedding
- Diet: The animais were offered maintenance diet for rats, mice and hamsters, SSNIFF R 10 mm pellet, SSNIFF-Versuchstierdiaten GmbH, D-4770 Soest, FRG.
- Water: Tap water ad libitum during the post-exposure observation period.

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12/12
Route of administration:
inhalation: aerosol
Type of inhalation exposure:
nose/head only
Vehicle:
air
Details on inhalation exposure:
TECHNICAL PROCEDURE:
- Exposure system: Head-nose inhalation system INA 20 (glass-steel construction, BASF Aktiengesellschaft, V ≈ 55 L); the animals are restrained in tubes and their snouts project into the inhalation chamber.
- Generator system: By means of a continuous infusion pump, INFU 362 (INDIGEL/Switzerland) and a two-component atomizer, mod. 970 (Schlick), a mixture of aerosol and air was generated.
- Experimental procedure: Constant amounts of the substance to be tested were supplied to a two-component atomizer by means of a metering pump. By means of compressed air (1.45 bar) an aerosol was generated, which was passed into the inhalation system. The exhaust air system was adjusted by 10% lower against the supply air system (positive pressure). This ensured that the mixture of test substance and air was not diluted by laboratory air in the breathing zones of the animals.

ANALYTICAL INVESTIGATIONS:
- Sampling: Apparatus: Quartz wool and a downstream fritted glass flask, BASF sampling station (with gas meter, impulse counter and automatic pump switching); Sorption solvent: 2-propanol; Sampling velocity: 1.25 m/s; Sampling amount: 1 L; Sampling site: immediately adjacent to the animal noses; Sampling probe ø: 7 mm; Sampling frequency: 1 sample every hour.
- Analytical method of determination: A gas-chromatographic method was used for the quantitative assay of the aerosol concentration. The aerosol samples were taken up in 40 mL 2-propanol (solvent). Gas-chromatographic method: GC HP/5840 A (Hewlett Packard). An internal standard was pipetted to the absorption sample contained in a 50 mL measuring flask and made up to the calibration mark. The chromatograpbic peak area was compared with calibration values and calculated.
- Particle size analysis: Equipment: Andersen Stack Sampler Mark III, Millipore vacuum compressed air pump XX 60 220 50, limiting orifice 3 L/min Millipore, sampling probe, internal diameter 6.9 mm, stopwatch. Procedure: 30 minutes after the beginning of the test at the earliest, one sample of the test group was taken for the particle size analysis. Before the sampling, the impactor was equipped with metal collecting discs and a backup particle filter. The impactor was connected to the pump and the test apparatus, and a sample (12 L) was removed. The impactor was taken apart, the collecting discs and the pre-impactor were rinsed with the solvent, the backup particle filter was eluted, and the samples obtained were analyzed by gas chromatography.
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
4.9 mg/L
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
Observation period: After the exposure period the surviving animals were observed for 14 days.
Clinical examinations: The body weight of the animals was checked before the beginning of the test, after 7 days and at the end of the observation period and was presented graphically. Clinical symptoms were recorded each workday. Mortality was checked each day.
Pathology: At the end of the 14-day observation period, the animals were sacrificed by CO2 and were subjected to a grosspathological examination.
Statistics:
The statistical evaluation of the concentration-response relationship was carried out in accordance with the binomial test (Wittig, H.: Mathematische Statistik 1974, pp. 32 - 35) according to tables of the BASF Computer Center.
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 4.9 mg/L air
Based on:
test mat.
Exp. duration:
4 h
Mortality:
No mortality was observed.
Clinical signs:
other: During exposure: aqueous discharge from noses, salivation. After exposure: aqueous, slight discharge from noses, salivation, reddened ears and limbs. After one day the animals were without findings.
Body weight:
The body weights of the test group were unimpaired on comparison to a control collective.
Gross pathology:
Organs: no abnormalities detected.
Other findings:
The MMAD 50% = 3.0 µm (geometrical standard deviation 2.9) was calculated from the results of the particle size analysis.
A fraction of 89% of the aerosol passing through the alveoli was obtained from the results of the particle size analysis.
Conclusions:
LC50: >4.9 mg/L on male / females.
Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LC50
Value:
4 900 mg/m³
Quality of whole database:
Read across from 3,7-dimethyloct-6-enenitrile (EC 257-288-8)

Acute toxicity: via dermal route

Link to relevant study records
Reference
Endpoint:
acute toxicity: dermal
Data waiving:
study scientifically not necessary / other information available
Justification for data waiving:
the study does not need to be conducted because the physicochemical and toxicological properties suggest no potential for a significant rate of absorption through the skin
Endpoint conclusion
Endpoint conclusion:
no study available

Additional information

Justification for classification or non-classification

In the CLP regulation acute toxicity means those adverse effects occuring following oral or dermal administration of a single dose of a substance or a mixture, or multiple doses given within 24 hours, or an inhalation exposure of 4 hours.

The substance does not meet the criteria for classification under the CLP regulations for acute toxicity via the oral route based on the result of an acute oral toxicity study, which gave a LD50 of 4490 mg/kg bodyweight, which is above the classification cut-off value ( ≤2000 mg/kg bodyweight) for acute oral toxicity.

In an acute inhalation toxicity study, a testing concentration of 4.9 mg/L air produced no mortalities and LC50 is >4.9 mg/L. The substance is therefore not classified for acute inhalation toxicity.