Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
Version / remarks:
1984 version
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Type:
Constituent
Type:
Constituent
Type:
impurity
Type:
impurity
Type:
impurity
Test material form:
liquid

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Activated sludge collected from the sewage treatment plant of CH - 4153 Reinach on 30/05/88.

Study design

Test type:
static
Water media type:
freshwater
Remarks:
dechlorinated drinking water
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
20 +/- 2 degrees C
Nominal and measured concentrations:
100 mg/L
Details on test conditions:
250 ml BOD fasks with gas inlet
Reference substance (positive control):
yes
Remarks:
3,5-dichlorphenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks on result:
not determinable because of methodological limitations
Remarks:
no effect on growth inhibition
Details on results:
Oxygen consumption per hour measured with ORION - Electrode Type 97-08 and plotted on a recorder.
Results with reference substance (positive control):
IC50 = 14 mg/L

Applicant's summary and conclusion

Conclusions:
There was no effect on microbial growth in activated sludge at 100 mg/L based on respiration. EC50>100 mg/L.
Executive summary:

There was no effect on microbial growth in activated sludge at 100 mg/L based on respiration. EC50>100 mg/L.