Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
Version / remarks:
1984 version
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Didodecyl nonylphenyl phosphite
EC Number:
252-457-2
EC Name:
Didodecyl nonylphenyl phosphite
Cas Number:
35239-35-1
Molecular formula:
C39-H73-O3-P
IUPAC Name:
Phosphorous acid, didodecyl nonylphenyl ester
Constituent 2
Chemical structure
Reference substance name:
Dodecyl bis(4-nonylphenyl) phosphite
EC Number:
284-117-4
EC Name:
Dodecyl bis(4-nonylphenyl) phosphite
Cas Number:
84787-76-8
Molecular formula:
C42-H71-O3-P
IUPAC Name:
Phosphorous acid, dodecyl bis(4-nonylphenyl) ester
Constituent 3
Chemical structure
Reference substance name:
4-nonylphenyl ditridecyl phosphite
EC Number:
284-119-5
EC Name:
4-nonylphenyl ditridecyl phosphite
Cas Number:
84787-77-9
Molecular formula:
C41-H77-O3-P
IUPAC Name:
Phosphorous acid, 4-nonylphenyl ditridecyl ester
Constituent 4
Chemical structure
Reference substance name:
Bis(4-nonylphenyl) tridecyl phosphite
EC Number:
284-120-0
EC Name:
Bis(4-nonylphenyl) tridecyl phosphite
Cas Number:
84787-78-0
Molecular formula:
C43-H73-O3-P
IUPAC Name:
Phosphorous acid, bis(4-nonylphenyl) tridecyl ester
impurity 1
Reference substance name:
Tris(nonylphenyl) phosphite
EC Number:
247-759-6
EC Name:
Tris(nonylphenyl) phosphite
Cas Number:
26523-78-4
IUPAC Name:
tris(2-nonylphenyl) phosphite
impurity 2
Reference substance name:
Didodecyl phosphonate
EC Number:
244-325-8
EC Name:
Didodecyl phosphonate
Cas Number:
21302-09-0
Molecular formula:
C24H51O3P
IUPAC Name:
Di(n-dodecyl)hydrogen phosphate
impurity 3
Chemical structure
Reference substance name:
Phenol, 4-nonyl-, branched
EC Number:
284-325-5
EC Name:
Phenol, 4-nonyl-, branched
Cas Number:
84852-15-3
Molecular formula:
C15H24O
IUPAC Name:
4-Nonylphenol, branched
Test material form:
liquid

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Activated sludge collected from the sewage treatment plant of CH - 4153 Reinach on 30/05/88.

Study design

Test type:
static
Water media type:
freshwater
Remarks:
dechlorinated drinking water
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
20 +/- 2 degrees C
Nominal and measured concentrations:
100 mg/L
Details on test conditions:
250 ml BOD fasks with gas inlet
Reference substance (positive control):
yes
Remarks:
3,5-dichlorphenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks on result:
not determinable because of methodological limitations
Remarks:
no effect on growth inhibition
Details on results:
Oxygen consumption per hour measured with ORION - Electrode Type 97-08 and plotted on a recorder.
Results with reference substance (positive control):
IC50 = 14 mg/L

Applicant's summary and conclusion

Conclusions:
There was no effect on microbial growth in activated sludge at 100 mg/L based on respiration. EC50>100 mg/L.
Executive summary:

There was no effect on microbial growth in activated sludge at 100 mg/L based on respiration. EC50>100 mg/L.