Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN-Vorschrift 38412 - L15
Version / remarks:
Static procedure
Deviations:
yes
Remarks:
Highest vehicle concentration: 950 mg/L Fish size: 35-50min (average: 44min) . Test volume: 15 L. Fish were not fed for 3 days prior to exposure.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Type:
Constituent
Type:
Constituent
Type:
impurity
Type:
impurity
Type:
impurity
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
5.0 g TK 12437 (CH 302) were mixed with and made up to 50 ml with DMF.

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
Golden orfe
22 days of acclimation
Length: 44 mm, average (35-50 mm)
Weight: 0.59 g, average (0.29-0.85 g)

Study design

Test type:
static
Water media type:
freshwater
Remarks:
Dechlorinated tap water (carbon filter)
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
254 mg CaC03/L
14.2 degrees d
Mol Ca/Mg: approx. 4/1
Test temperature:
20 +/- 1 degrees C
pH:
7.9-8.1
Dissolved oxygen:
88-98%
Nominal and measured concentrations:
Test concentrations 5.8, 10, 18, 32, 58, 100
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
1 mortality in 10 at 5.8 mg/L, complete mortality (10 in 10) at higher concentrations

Applicant's summary and conclusion

Conclusions:
An EC50 of 7.1 mg/L was determined based on the mortality results.
Executive summary:

An EC50 of 7.1 mg/L was determined based on the mortality results.