Reaction Mass of Didodecyl nonylphenyl phosphite and Dodecyl bis(4-nonylphenyl) phosphite and 4-nonylphenyl ditridecyl phosphite and Bis(4-nonylphenyl) tridecyl

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN-Vorschrift 38412 - L15

Version / remarks:

Static procedure

Deviations:

yes

Remarks:

Highest vehicle concentration: 950 mg/L Fish size: 35-50min (average: 44min) . Test volume: 15 L. Fish were not fed for 3 days prior to exposure.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Didodecyl nonylphenyl phosphite

EC Number:

252-457-2

EC Name:

Didodecyl nonylphenyl phosphite

Cas Number:

35239-35-1

Molecular formula:

C39-H73-O3-P

IUPAC Name:

Phosphorous acid, didodecyl nonylphenyl ester

Constituent 2

Reference substance name:

Dodecyl bis(4-nonylphenyl) phosphite

EC Number:

284-117-4

EC Name:

Dodecyl bis(4-nonylphenyl) phosphite

Cas Number:

84787-76-8

Molecular formula:

C42-H71-O3-P

IUPAC Name:

Phosphorous acid, dodecyl bis(4-nonylphenyl) ester

Constituent 3

Reference substance name:

4-nonylphenyl ditridecyl phosphite

EC Number:

284-119-5

EC Name:

4-nonylphenyl ditridecyl phosphite

Cas Number:

84787-77-9

Molecular formula:

C41-H77-O3-P

IUPAC Name:

Phosphorous acid, 4-nonylphenyl ditridecyl ester

Constituent 4

Reference substance name:

Bis(4-nonylphenyl) tridecyl phosphite

EC Number:

284-120-0

EC Name:

Bis(4-nonylphenyl) tridecyl phosphite

Cas Number:

84787-78-0

Molecular formula:

C43-H73-O3-P

IUPAC Name:

Phosphorous acid, bis(4-nonylphenyl) tridecyl ester

impurity 1

Reference substance name:

Tris(nonylphenyl) phosphite

EC Number:

247-759-6

EC Name:

Tris(nonylphenyl) phosphite

Cas Number:

26523-78-4

IUPAC Name:

tris(2-nonylphenyl) phosphite

impurity 2

Reference substance name:

Didodecyl phosphonate

EC Number:

244-325-8

EC Name:

Didodecyl phosphonate

Cas Number:

21302-09-0

Molecular formula:

C24H51O3P

IUPAC Name:

Di(n-dodecyl)hydrogen phosphate

impurity 3

Reference substance name:

Phenol, 4-nonyl-, branched

EC Number:

284-325-5

EC Name:

Phenol, 4-nonyl-, branched

Cas Number:

84852-15-3

Molecular formula:

C15H24O

IUPAC Name:

4-Nonylphenol, branched

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

5.0 g TK 12437 (CH 302) were mixed with and made up to 50 ml with DMF.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

Golden orfe
22 days of acclimation
Length: 44 mm, average (35-50 mm)
Weight: 0.59 g, average (0.29-0.85 g)

Study design

Test type:

static

Water media type:

freshwater

Remarks:

Dechlorinated tap water (carbon filter)

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

254 mg CaC03/L
14.2 degrees d
Mol Ca/Mg: approx. 4/1

Test temperature:

20 +/- 1 degrees C

pH:

7.9-8.1

Dissolved oxygen:

88-98%

Nominal and measured concentrations:

Test concentrations 5.8, 10, 18, 32, 58, 100

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

1 mortality in 10 at 5.8 mg/L, complete mortality (10 in 10) at higher concentrations

Applicant's summary and conclusion

Conclusions:

An EC50 of 7.1 mg/L was determined based on the mortality results.

Executive summary:

An EC50 of 7.1 mg/L was determined based on the mortality results.