Reaction Mass of Didodecyl nonylphenyl phosphite and Dodecyl bis(4-nonylphenyl) phosphite and 4-nonylphenyl ditridecyl phosphite and Bis(4-nonylphenyl) tridecyl

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.5 (Degradation: Biochemical Oxygen Demand)

Version / remarks:

84/449/EEC C.5
Biotic degradation: modified Sturm test

Deviations:

yes

Remarks:

1.5 L vessels rather than 3 L vessels

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Didodecyl nonylphenyl phosphite

EC Number:

252-457-2

EC Name:

Didodecyl nonylphenyl phosphite

Cas Number:

35239-35-1

Molecular formula:

C39-H73-O3-P

IUPAC Name:

Phosphorous acid, didodecyl nonylphenyl ester

Constituent 2

Reference substance name:

Dodecyl bis(4-nonylphenyl) phosphite

EC Number:

284-117-4

EC Name:

Dodecyl bis(4-nonylphenyl) phosphite

Cas Number:

84787-76-8

Molecular formula:

C42-H71-O3-P

IUPAC Name:

Phosphorous acid, dodecyl bis(4-nonylphenyl) ester

Constituent 3

Reference substance name:

4-nonylphenyl ditridecyl phosphite

EC Number:

284-119-5

EC Name:

4-nonylphenyl ditridecyl phosphite

Cas Number:

84787-77-9

Molecular formula:

C41-H77-O3-P

IUPAC Name:

Phosphorous acid, 4-nonylphenyl ditridecyl ester

Constituent 4

Reference substance name:

Bis(4-nonylphenyl) tridecyl phosphite

EC Number:

284-120-0

EC Name:

Bis(4-nonylphenyl) tridecyl phosphite

Cas Number:

84787-78-0

Molecular formula:

C43-H73-O3-P

IUPAC Name:

Phosphorous acid, bis(4-nonylphenyl) tridecyl ester

impurity 1

Reference substance name:

Tris(nonylphenyl) phosphite

EC Number:

247-759-6

EC Name:

Tris(nonylphenyl) phosphite

Cas Number:

26523-78-4

IUPAC Name:

tris(2-nonylphenyl) phosphite

impurity 2

Reference substance name:

Didodecyl phosphonate

EC Number:

244-325-8

EC Name:

Didodecyl phosphonate

Cas Number:

21302-09-0

Molecular formula:

C24H51O3P

IUPAC Name:

Di(n-dodecyl)hydrogen phosphate

impurity 3

Reference substance name:

Phenol, 4-nonyl-, branched

EC Number:

284-325-5

EC Name:

Phenol, 4-nonyl-, branched

Cas Number:

84852-15-3

Molecular formula:

C15H24O

IUPAC Name:

4-Nonylphenol, branched

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Bacteria collected from activated sludge of the sewage treatment plant of CH-4153 Reinach on 03/08/92. The pH after collection was 7.1 .

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

12.4 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

22.4 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

Vessels : 2 Liter flasks (dark brown glass), equipped with gas inlet and magnetic stirrer
Temperature: 22 +/- 2 degrees C
Duration: 28 days
Air: 25 ml/min purified from carbon dioxide.

Reference substance

Reference substance:

other: not specified but performed as expected

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

25

Sampling time:

28 d

Details on results:

Theorteical CO2 pased on 12.4 mg/1 = 51.41 mg CO2/I.5 liter

Applicant's summary and conclusion

Interpretation of results:

not readily biodegradable

Conclusions:

There was 25% degradation after 28-days. After an inital lag in degradation, there was significant degradation during the final week of the study suggesting the test material would have continued to degrade.

Executive summary:

There was 25% degradation after 28-days.  After an inital lag in biodegradation, there was significant biodegradation during the final week of the study suggesting the test material would have continued to degrade.