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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-[N-(2-cyanoethyl)-4-[(2,6-dichloro-4-nitrophenyl)azo]anilino]ethyl acetate
EC Number:
226-070-4
EC Name:
2-[N-(2-cyanoethyl)-4-[(2,6-dichloro-4-nitrophenyl)azo]anilino]ethyl acetate
Cas Number:
5261-31-4
Molecular formula:
C19H17Cl2N5O4
IUPAC Name:
2-[(2-cyanoethyl)({4-[2-(2,6-dichloro-4-nitrophenyl)diazen-1-yl]phenyl}) amino]ethyl acetate
Test material form:
solid: particulate/powder
Remarks:
orange powder

Study design

Inoculum or test system:
not specified
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
6 mg/L
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Key result
Parameter:
% degradation (O2 consumption)
Value:
5
Sampling time:
28 d
Remarks on result:
other: not readily biodegradable

BOD5 / COD results

Results with reference substance:
Aniline (28 d, ready biodegradability %): 78

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Under the study conditions, the test substance biodegradation was equivalent to 5% after 28 d. The test substance was therefore not considered to be readily biodegradable.
Executive summary:

A study was conducted to determine the ready biodegradability of the test substance according to OECD Guideline 301 D. The inoculum (6 mg/L) was exposed for 28 d to the test substance. The oxygen demand was monitored for 28 d. The test setup also included the reference substance aniline. The biodegradation of the reference substance after 14 d was determined to be 75%. Under the study conditions, the test substance biodegradation was equivalent to 5% after 28 d. The test substance was therefore not considered to be readily biodegradable (Mueller, 1993).