Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 222-671-0 | CAS number: 3570-55-6
Toxicological information
Skin sensitisation
Currently viewing:
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 Mar 1992 - 02 Feb 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-6 (Skin Sensitisation)
- Deviations:
- no
- GLP compliance:
- yes
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- The LLNA was not yet validated at the time of the study.
Test material
- Reference substance name:
- 2,3-bis((2-mercaptoethyl)thio)-1-propanethiol
- EC Number:
- 411-290-7
- EC Name:
- 2,3-bis((2-mercaptoethyl)thio)-1-propanethiol
- Cas Number:
- 131538-00-6
- Molecular formula:
- C7 H16 S5
- IUPAC Name:
- 2,3-bis((2-mercaptoethyl)thio)-1-propanethiol
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Pirbright-Hartley
- Sex:
- male
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source:
Winkelmann, Borchen, Germany
- Weight at study initiation:
test group: 342.6 ± 19.63 g; vehicle controls: 342.3 ± 17.62 g
- Housing:
1 - 4 per cage, Makrolon Type IV
- Diet (e.g. ad libitum):
Altromin tpf 3022, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Quarantine: 6 day
ENVIRONMENTAL CONDITIONS
- Temperature (°C):
21 ± 2 °C
- Humidity (%): 45 - 65%
- Air changes (per hr): 12 - 15
- Photoperiod (hrs dark / hrs light):
12 / 12
- IN-LIFE DATES: From: 1992-08-04 To: 1992-09-24
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal
- Vehicle:
- paraffin oil
- Concentration / amount:
- 0.2%
- Day(s)/duration:
- Day 0
- Adequacy of induction:
- highest concentration used causing mild-to-moderate skin irritation and well-tolerated systemically
- Route:
- epicutaneous, occlusive
- Vehicle:
- petrolatum
- Concentration / amount:
- 1.0%
- Day(s)/duration:
- Day 7, 48 h
- Adequacy of induction:
- highest concentration used causing mild-to-moderate skin irritation and well-tolerated systemically
Challenge
- No.:
- #1
- Route:
- epicutaneous, occlusive
- Vehicle:
- petrolatum
- Concentration / amount:
- 0.005%
- Day(s)/duration:
- Day 21, 24 h
- Adequacy of challenge:
- highest non-irritant concentration
- No. of animals per dose:
- 22
- Details on study design:
- RANGE FINDING TESTS:
Intradermal: 0.2%, 1%, 5%, 10% w/w in paraffin oil
Topical: 0.005%, 0.01%, 0.05%, 0.2%, 1%, 4%, 10%, 25% w/w in petrolatum - Challenge controls:
- 22 naive (sham-induced) animals
- Positive control substance(s):
- yes
- Remarks:
- DNCB, 0.1% (ethanol), 1.0% (petrolatum), 0.025% (petrolatum) for intradermal induction, topical induction, and challenge, respectively.
Results and discussion
- Positive control results:
- 22/22 of animals showed skin reactions
In vivo (non-LLNA)
Resultsopen allclose all
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 0.005%
- No. with + reactions:
- 0
- Total no. in group:
- 22
- Clinical observations:
- none
- Key result
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 0.005%
- No. with + reactions:
- 0
- Total no. in group:
- 22
- Clinical observations:
- none
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 0.005%
- No. with + reactions:
- 0
- Total no. in group:
- 22
- Clinical observations:
- none
- Remarks on result:
- no indication of skin sensitisation
- Key result
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 0.005%
- No. with + reactions:
- 0
- Total no. in group:
- 22
- Clinical observations:
- none
- Remarks on result:
- no indication of skin sensitisation
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- positive control
- Dose level:
- 0.025%
- No. with + reactions:
- 22
- Total no. in group:
- 22
- Clinical observations:
- none
- Remarks on result:
- positive indication of skin sensitisation
- Key result
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- positive control
- Dose level:
- 0.025%
- No. with + reactions:
- 22
- Total no. in group:
- 22
- Clinical observations:
- none
- Remarks on result:
- positive indication of skin sensitisation
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Based on the GPMT results, DMPT is not a skin sensitizer.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
